Change search
ReferencesLink to record
Permanent link

Direct link
Altering the specificity of subtilisin B. lentus by combining site-directed mutagenesis and chemical modification
University of Toronto, Canada.ORCID iD: 0000-0002-9577-832X
Show others and affiliations
1996 (English)In: Bioorganic and Medicinal Chemistry Letters, ISSN 0960894X (ISSN), Vol. 6, no 21, 2507-2512 p.Article in journal (Refereed) Published
Abstract [en]

The thiol side chain of the M222C mutant of the subtilisin from Bacillus lentus (SBL) has been chemically modified by methyl-, aminoethyl-, and sulfonatoethylthiosulfonate reagents. Introduction of charged residues into the active site of the enzyme reduced the catalytic efficiency with Suc-AAPF-pNA as the substrate, but resulted in better binding of sterically demanding boronic acid inhibitors.

Place, publisher, year, edition, pages
1996. Vol. 6, no 21, 2507-2512 p.
Keyword [en]
boronic acid derivative, subtilisin, article, Eggerthella lenta, enzyme activity, enzyme modification, site directed mutagenesis, Bacillus lentus
National Category
Biocatalysis and Enzyme Technology
URN: urn:nbn:se:kth:diva-74867DOI: 10.1016/0960-894X(96)00467-2OAI: diva2:490126
References: Cornish, V.W., Mendel, D., Schultz, P.G., (1995) Angew. Chem., Int. Ed. Engl., 34, p. 621; Polgar, L., Bender, M.L., (1966) J. Am. Chem. Soc., 88, p. 3153; Neet, K.E., Koshland D.E., Jr., (1966) Proc. Natl. Acad. Sci. USA, 56, p. 1606; Hilvert, D., Kaiser, E.T., (1985) J. Am. Chem. Soc., 107, p. 5805; Radziejewski, C., Ballou, D.P., Kaiser, E.T., (1985) J. Am. Chem. Soc., 107, p. 3352; Stewart, K.D., Radziejewski, C., Kaiser, E.T., (1986) J. Am. Chem. Soc., 108, p. 3480; Rokita, S.E., Kaiser, E.T., (1986) J. Am. Chem. Soc., 108, p. 4984; Kokubo, T., Sassa, S., Kaiser, E.T., (1987) J. Am. Chem. Soc., 109, p. 606; Kaiser, E.T., (1988) Angew. Chem., Int. Ed. Engl., 27, p. 913; Hilvert, D., Hatanaka, Y., Kaiser, E.T., (1988) J. Am. Chem. Soc., 110, p. 682; Wu, Z.-P., Hilvert, D., (1989) J. Am. Chem. Soc., 111, p. 4514; Bell, I.M., Fisher, M.L., Wu, Z.-P., Hilvert, D., (1993) Biochemistry, 32, p. 3754; Peterson, E.B., Hilvert, D., (1995) Biochemistry, 34, p. 6616; Suckling, C.J., Zhu, L.-M., (1993) Bioorg. Med. Chem. Lett., 3, p. 531; Smith, D.J., Kenyon, G.L., (1974) J. Biol. Chem., 249, p. 3317; Kenyon, G.L., Bruice, T.W., (1977) Methods Enzymol., 47, p. 407; Brocklehurst, K., (1979) Int. J. Biochem, 10, p. 259; Bruice, T.W., Kenyon, G.L., (1982) J. Protein Chem., 1, p. 47; Wynn, R., Richards, F.M., (1995) Methods Enzymol., 257, p. 351; Bech, L.M., Breddam, K., (1988) Carlsberg Res. Commun., 53, p. 381; Wynn, R., Harkins, P.C., Richards, F.M., Fox, R.O., (1996) Protein Sci., 5, p. 1026; GrÞn, H., Bech, L.M., Branner, S., Breddam, K., (1990) Eur. J. Biochemistry, 194, p. 897; noteStabile, M.R., Lai, W.G., DeSantis, G., Gold, M., Jones, J.B., Mitchinson, C., Bott, R.R., Liu, C.-C., (1996) Bioorg. Med. Chem. Lett., , in press; noteCleland, W.W., (1964) Biochemistry, 3, p. 480; Graycar, T.P., Bott, R.R., Caldwell, R.M., Dauberman, J.L., Lad, P.J., Power, S.D., Sagar, I.H., Estell, D.A., (1992) Ann. N. Y. Acad. Sci., 672, p. 71; Hsia, C.Y., Ganshaw, G., Paech, C., Murray, C.J., (1996) Anal. Biochem., , in press; Waley, S.G., (1982) Biochem. J., 205, p. 631 NR 20140805Available from: 2012-02-03 Created: 2012-02-03 Last updated: 2012-02-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Berglund, P.
Biocatalysis and Enzyme Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 93 hits
ReferencesLink to record
Permanent link

Direct link