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Creation of a synthetically useful lipase with higher than wild-type enantioselectivity and maintained catalytic activity
KTH, School of Biotechnology (BIO), Biochemistry (closed 20130101).ORCID iD: 0000-0002-9577-832X
1999 (English)In: Organic Letters, ISSN 1523-7060, E-ISSN 1523-7052, Vol. 1, no 5, 763-765 p.Article in journal (Refereed) Published
Abstract [en]

Formula presented Wild type I: 89.9% ee (E=32) Wild type II: 79.8% ee (E=10) Lipase hybrid: 95.4% ee (E=54) We have found that two Geotrichum candidum lipase isozymes have remarkably different abilities to differentiate between enantiomers of ethyl 2-methyldecanoate. By rational recombination of selected portions of the two isozymes, we have created a novel lipase with an enantioselectivity superior to that of the best wild-type parent isozyme. Site-directed mutagenesis identified two key amino acid residues responsible for the improved enantioselectivity without compromised total activity of the reengineered enzyme.

Place, publisher, year, edition, pages
1999. Vol. 1, no 5, 763-765 p.
Keyword [en]
amino acid, decanoic acid derivative, ethyl 2 methyldecanoate, ethyl-2-methyldecanoate, isoenzyme, triacylglycerol lipase, article, catalysis, chemistry, enzyme specificity, enzymology, Geotrichum, site directed mutagenesis, stereoisomerism, synthesis, Amino Acids, Decanoates, Isoenzymes, Lipase, Mutagenesis, Site-Directed, Substrate Specificity
National Category
Biocatalysis and Enzyme Technology
URN: urn:nbn:se:kth:diva-74863OAI: diva2:490134

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