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Efficient bioreduction of bicyclo[2.2.2]octane-2,5-dione and bicyclo[2.2.2]oct-7-ene-2,5-dione by genetically engineered Saccharomyces cerevisiae
Division of Organic Chemistry, Center for Chemistry and Chemical Engineering, Lund University.ORCID iD: 0000-0003-1933-4193
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2006 (English)In: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 4, no 11, 2304-2312 p.Article in journal (Refereed) Published
Abstract [en]

A screening of non-conventional yeast species and several Saccharomyces cerevisiae ( baker's yeast) strains overexpressing known carbonyl reductases revealed the S. cerevisiae reductase encoded by YMR226c as highly efficient for the reduction of the diketones 1 and 2 to their corresponding hydroxyketones 3 - 6 ( Scheme 1) in excellent enantiomeric excesses. Bioreduction of 1 using the genetically engineered yeast TMB4100, overexpressing YMR226c, resulted in > 99% ee for hydroxyketone (+)- 4 and 84 - 98% ee for (-)- 3, depending on the degree of conversion. Baker's yeast reduction of diketone 2 resulted in > 98% ee for the hydroxyketones (+)- 5 and (+)- 6. However, TMB4100 led to significantly higher conversion rates ( over 40 fold faster) and also a minor improvement of the enantiomeric excesses (> 99%).

Place, publisher, year, edition, pages
2006. Vol. 4, no 11, 2304-2312 p.
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Organic Chemistry
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URN: urn:nbn:se:kth:diva-79155DOI: 10.1039/b603500kISI: 000237907500025OAI: oai:DiVA.org:kth-79155DiVA: diva2:495176
Note
QC 20120221Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2017-12-07Bibliographically approved

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Franzén, Johan

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