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Studying Individual Events in Biology
Laboratory of Cellular Biophysics, Rockefeller University, New York, USA.ORCID iD: 0000-0002-1850-5440
Laboratory of Cellular Biophysics, Rockefeller University, New York, USA.
2007 (English)In: Annual Review of Biochemistry, ISSN 0066-4154, E-ISSN 1545-4509, Vol. 76, 419-446 p.Article in journal (Refereed) Published
Abstract [en]

Studying the properties of individual events and molecules offers a host of advantages over taking only macroscopic measurements of populations. Here we review such advantages, as well as some pitfalls, focusing on examples from biological imaging. Examples include single proteins, their interactions in cells, organelles, and their interactions both with each other and with parts of the cell. Additionally, we discuss constraints that limit the study of single events, along with the criteria that must be fulfilled to determine whether single molecules or events are being detected.

Place, publisher, year, edition, pages
Annual Reviews , 2007. Vol. 76, 419-446 p.
Keyword [en]
endocytosis, exocytosis, imaging, quantum dots, single molecule
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:kth:diva-79191DOI: 10.1146/annurev.biochem.76.062305.094225ISI: 000249336800019OAI: oai:DiVA.org:kth-79191DiVA: diva2:495228
Note
QC 20120209Available from: 2012-02-09 Created: 2012-02-08 Last updated: 2017-12-07Bibliographically approved

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Wennmalm, Stefan

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