Change search
ReferencesLink to record
Permanent link

Direct link
[ADH and cAMP receptor binding in the kidney medulla of mice insensitive to ADH].
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.
1991 (Russian)In: Fiziologicheskiń≠ zhurnal SSSR imeni I. M. Sechenova, ISSN 0015-329X, Vol. 77, no 5, 85-91 p.Article in journal (Refereed) Published
Abstract [ru]

Specific binding of ADH by the membrane fraction of the kidney medulla was lower in the normal CBA mice than in mutant mice with nephrogenic diabetes. Gel filtration of the solubilized ADH receptors of mutants revealed the presence of an unidentified factor which caused cooperative binding of ADH. DEAE-chromatography revealed no difference between cytosolic cAMP receptors in normal and mutant animals. Assay of GTP-ase activity of the membrane fraction revealed that ADH increased this parameter in CBA mice but not in mutant animals. Cholera toxin significantly diminished membrane ATP-ase activity whereas membrane preparations from mutant mice developed a reactivity to ADH. GTP binding ability in these preparations was higher than inn intact ones. In CBA mice this ability increased dramatically. HPLC profiles of G-protein complexes with GNP were very different in CBA and mutant mice. Mutation seems to cause changes both in binding and in "cross-talk" link op-complex membrane receptor of ADH.

Place, publisher, year, edition, pages
1991. Vol. 77, no 5, 85-91 p.
National Category
Cell Biology
URN: urn:nbn:se:kth:diva-80474PubMedID: 1666385OAI: diva2:496361
NR 20140805Available from: 2012-02-09 Created: 2012-02-09 Last updated: 2012-02-09Bibliographically approved

Open Access in DiVA

No full text

By organisation
Cell Physics
Cell Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 38 hits
ReferencesLink to record
Permanent link

Direct link