Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Community proteogenomics highlights microbial strain-variant protein expression within activated sludge performing enhanced biological phosphorus removal
Department of Earth and Planetary Sciences, University of California at Berkeley.ORCID iD: 0000-0002-3627-6899
Show others and affiliations
2008 (English)In: ISME JOURNAL, ISSN 1751-7362, Vol. 2, no 8, 853-864 p.Article in journal (Refereed) Published
Abstract [en]

Enhanced biological phosphorus removal (EBPR) selects for polyphosphate accumulating microorganisms to achieve phosphate removal from wastewater. We used high-resolution community proteomics to identify key metabolic pathways in 'Candidatus Accumulibacter phosphatis' (A. phosphatis)-mediated EBPR and to evaluate the contributions of co-existing strains within the dominant population. Overall, 702 proteins from the A. phosphatis population were identified. Results highlight the importance of denitrification, fatty acid cycling and the glyoxylate bypass in EBPR. Strong similarity in protein profiles under anaerobic and aerobic conditions was uncovered (only 3% of A. phosphatis-associated proteins exhibited statistically significant abundance differences). By comprehensive genome-wide alignment of 13,930 orthologous proteins, we uncovered substantial differences in protein abundance for enzyme variants involved in both core-metabolism and EBPR-specific pathways among the A. phosphatis population. These findings suggest an essential role for genetic diversity in maintaining the stable performance of EBPR systems and, hence, demonstrate the power of integrated cultivation-independent genomics and proteomics for the analysis of complex biotechnological systems.

Place, publisher, year, edition, pages
2008. Vol. 2, no 8, 853-864 p.
Keyword [en]
Aerobiosis, Anaerobiosis, Bacteria/classification/*genetics/*metabolism, Bacterial Proteins/analysis/genetics, DNA, Bacterial/chemistry/genetics, Fatty Acids/metabolism, Genetic Variation, Glyoxylates/metabolism, Molecular Sequence Data, Nitrogen/metabolism, Phosphorus/*metabolism, Proteome/*analysis/*genetics, Sewage/*microbiology
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:kth:diva-80959DOI: 10.1038/ismej.2008.38ISI: 000258267700007ISBN: 1751-7370 (Electronic) 1751-7362 (Linking) (print)OAI: oai:DiVA.org:kth-80959DiVA: diva2:496989
Note
QC 20120214Available from: 2012-02-10 Created: 2012-02-10 Last updated: 2012-02-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Andersson, Anders F.
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
isbn
urn-nbn

Altmetric score

doi
isbn
urn-nbn
Total: 27 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf