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Performance of a 70-mer oligonucleotide microarray for genotyping of Campylobacter jejuni
Swedish Institute for Infectious Disease Control, SE-17182 Solna, Sweden. Limnology/Department of Ecology and Evolution, Uppsala University .
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2008 (English)In: BMC Microbiology, ISSN 1471-2180, Vol. 8Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Campylobacter jejuni is widespread in the environment and is the major cause of bacterial gastroenteritis in humans. In the present study we use microarray-based comparative genomic hybridizations (CGH), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to analyze closely related C. jejuni isolates from chicken and human infection. RESULTS: With the exception of one isolate, the microarray data clusters the isolates according to the five groups determined by PFGE. In contrast, MLST defines only three genotypes among the isolates, indicating a lower resolution. All methods show that there is no inherit difference between isolates infecting humans and chicken, suggesting a common underlying population of C. jejuni. We further identify regions that frequently differ between isolates, including both previously described and novel regions. Finally, we show that genes that belong to certain functional groups differ between isolates more often than expected by chance. CONCLUSION: In this study we demonstrated the utility of 70-mer oligonucleotide microarrays for genotyping of Campylobacter jejuni isolates, with resolution outperforming MLST.

Place, publisher, year, edition, pages
2008. Vol. 8
Keyword [en]
Animals, Bacterial Typing Techniques, Campylobacter Infections/*microbiology/*veterinary, Campylobacter jejuni/*classification/*genetics/iso, Chickens/microbiology, DNA, Bacterial/analysis/genetics, Electrophoresis, Gel, Pulsed-Field, Genotype, Humans, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis/*methods, Poultry Diseases/*microbiology, Sequence Analysis, DNA
National Category
Natural Sciences
URN: urn:nbn:se:kth:diva-80958DOI: 10.1186/1471-2180-8-73ISI: 000256297800001OAI: diva2:496997
QC 20120213Available from: 2012-02-10 Created: 2012-02-10 Last updated: 2012-02-13Bibliographically approved

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Andersson, Anders F.
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