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Method for amplification
KTH, School of Biotechnology (BIO), Gene Technology.ORCID iD: 0000-0003-4313-1601
2005 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

The invention refers to a method for multiplex amplification of at least one specific nucleic acid locus, comprising the steps of: providing at least one oligonucleotide probe pair that is designed so that the first and second probe of the pair anneal to a specific nucleic acid locus on a target molecule, in which pair the first probe has an extendable 3'-end, and a second probe has a 5'-end that is directly or indirectly labelled with a phosphate group; providing a target molecule comprising at least one specific nucleic acid locus; allowing the probe pair to anneal to the target molecule; allowing the 3'-end of the first probe to extend by influence of polymerase by adding a set of three different dNTPs; ligating the 3'-end of the extended first probe to the 5'-end of the second probe. Hereby, a method is provided which allows a high specificity for simultaneous amplification of several loci. Further, the invention involves a kit for use in the method of the invention.

Place, publisher, year, edition, pages
2005.
Keyword [en]
amplification nucleic acids
National Category
Biological Sciences
Research subject
SRA - Molecular Bioscience
Identifiers
URN: urn:nbn:se:kth:diva-82379OAI: oai:DiVA.org:kth-82379DiVA: diva2:498170
Patent
EP 1874957A1
Note

QS 2015

Available from: 2012-02-11 Created: 2012-02-11 Last updated: 2015-01-27Bibliographically approved

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