Method for amplification
2005 (English)Patent (Other (popular science, discussion, etc.))
The invention refers to a method for multiplex amplification of at least one specific nucleic acid locus, comprising the steps of: providing at least one oligonucleotide probe pair that is designed so that the first and second probe of the pair anneal to a specific nucleic acid locus on a target molecule, in which pair the first probe has an extendable 3'-end, and a second probe has a 5'-end that is directly or indirectly labelled with a phosphate group; providing a target molecule comprising at least one specific nucleic acid locus; allowing the probe pair to anneal to the target molecule; allowing the 3'-end of the first probe to extend by influence of polymerase by adding a set of three different dNTPs; ligating the 3'-end of the extended first probe to the 5'-end of the second probe. Hereby, a method is provided which allows a high specificity for simultaneous amplification of several loci. Further, the invention involves a kit for use in the method of the invention.
Place, publisher, year, edition, pages
amplification nucleic acids
Research subject SRA - Molecular Bioscience
IdentifiersURN: urn:nbn:se:kth:diva-82379OAI: oai:DiVA.org:kth-82379DiVA: diva2:498170
QS 20152012-02-112012-02-112015-01-27Bibliographically approved