Change search
ReferencesLink to record
Permanent link

Direct link
Method for amplification
KTH, School of Biotechnology (BIO), Gene Technology.
2005 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

The invention refers to a method for multiplex amplification of at least one specific nucleic acid locus, comprising the steps of: providing at least one oligonucleotide probe pair that is designed so that the first and second probe of the pair anneal to a specific nucleic acid locus on a target molecule, in which pair the first probe has an extendable 3'-end, and a second probe has a 5'-end that is directly or indirectly labelled with a phosphate group; providing a target molecule comprising at least one specific nucleic acid locus; allowing the probe pair to anneal to the target molecule; allowing the 3'-end of the first probe to extend by influence of polymerase by adding a set of three different dNTPs; ligating the 3'-end of the extended first probe to the 5'-end of the second probe. Hereby, a method is provided which allows a high specificity for simultaneous amplification of several loci. Further, the invention involves a kit for use in the method of the invention.

Place, publisher, year, edition, pages
Keyword [en]
amplification nucleic acids
National Category
Biological Sciences
Research subject
SRA - Molecular Bioscience
URN: urn:nbn:se:kth:diva-82379OAI: diva2:498170
EP 1874957A1

QS 2015

Available from: 2012-02-11 Created: 2012-02-11 Last updated: 2015-01-27Bibliographically approved

Open Access in DiVA

No full text

Other links

Search in DiVA

By author/editor
Lundeberg, Joakim
By organisation
Gene Technology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 82 hits
ReferencesLink to record
Permanent link

Direct link