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Chemical method for the analysis of DNA sequences
1993 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

The invention provides a method of identification of the base in a target position in a DNA sequence wherein sample DNA is subjected to amplification; the amplified DNA is immobilised and then subjected to strand separation, the non-immobilised strand being removed and an extension primer, which hybridises to the immobilised DNA immediately adjacent to the target position, is provided; each of four aliquots of the immobilised single stranded DNA is then subjected to a polymerase reaction in the presence of a dideoxynucleotide, each aliquot using a different dideoxynucleotide whereby only the dideoxynucleotide whereby only the dideoxynucleotide complementary to the base in the target position becomes incorpored; the four aliquots are then subjected to extension in the presence of all four deoxynucleotides, whereby in each aliquot the DNA which has not reacted with the dideoxynucleotide is extended to form double stranded DNA while the dideoxy-blocked DNA remains as non-extended DNA; followed by identification of the double stranded and/or non-extended DNA to indicate which dideoxynucleotide was incorporated and hence which base was present in the target position.

Place, publisher, year, edition, pages
Keyword [en]
detection of immobilized nucleic acids
National Category
Biological Sciences
Research subject
SRA - Molecular Bioscience
URN: urn:nbn:se:kth:diva-82392OAI: diva2:498190
AU 669365B (1996-06-06)

US 5534424 A (1996-07-09);  DE 69307503 D1 (1997-02-27); JP 03421036 B2 (2003-06-30); CA 2135607 C (2005-08-02)

Available from: 2012-02-11 Created: 2012-02-11 Last updated: 2015-01-23Bibliographically approved

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Lundeberg, JoakimUhlén, Mathias
Biological Sciences

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