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The role of lipid composition for insertion and stabilization of amino acids in membranes.
Stockholm University.
Stockholm University.ORCID iD: 0000-0002-2734-2794
2009 (English)In: Journal of Chemical Physics, ISSN 0021-9606, E-ISSN 1089-7690, Vol. 130, no 18, 185101- p.Article in journal (Refereed) Published
Abstract [en]

While most membrane protein helices are clearly hydrophobic, recent experiments have indicated that it is possible to insert marginally hydrophobic helices into bilayers and have suggested apparent in vivo free energies of insertion for charged residues that are low, e.g., a few kcals for arginine. In contrast, a number of biophysical simulation studies have predicted that the bilayer interior is close to a pure hydrophobic environment with large penalties for hydrophilic amino acids--and yet the experimental scales do significantly better at predicting actual membrane proteins from sequence. Here, we have systematically studied the dependence of the free energy profiles on lipid properties, including tail length, saturation, headgroup hydrogen bond strength, and charge, both to see to whether the in vivo insertion can be explained in whole or part from lipid composition of the endoplasmic reticulum (ER) membranes, and if the solvation properties can help interpret how protein function depends on the lipids. We find that lipid charge is important to stabilize charged amino acids inside the bilayer (with implications, e.g., for ion channels), that thicker bilayers have higher solvation costs for hydrophilic side chains, and that headgroup hydrogen bond strength determines how adaptive the lipids are as a hydrophobic/hydrophilic solvent. None of the different free energy profiles are even close to the low apparent in vivo insertion cost, which suggests that regardless of the specific ER membrane composition the current experimental results cannot be explained by normal lipid-type variation.

Place, publisher, year, edition, pages
2009. Vol. 130, no 18, 185101- p.
National Category
Theoretical Chemistry
URN: urn:nbn:se:kth:diva-82595DOI: 10.1063/1.3129863ISI: 000266263200054PubMedID: 19449954OAI: diva2:498390
QC 20120213Available from: 2012-02-12 Created: 2012-02-12 Last updated: 2012-02-13Bibliographically approved

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Lindahl, Erik
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