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Biosynthetic enzymes for (1,3)-β-glucans and (1,3;1,6)-β-glucans in protozoans and chromistans. biochemical characterization and molecular biology
KTH, School of Biotechnology (BIO), Glycoscience.
2009 (English)In: Chemistry, biochemistry and biology of 1,3-beta-glucans and related polysaccharides / [ed] A. Bacic, B.A. Stone and G.B. Fincher, Elsevier, 2009, 233-258 p.Chapter in book (Refereed)
Abstract [en]

This chapter discusses the biochemical characterization and molecular biology of biosynthetic enzymes for (1,3)-β-glucans and (1,3:1,6)-β-Glucans in Protozoans and Chromistans. The biosynthesis of linear β-glucans is generally considered to involve several steps. The process starts with the synthesis of an initiator of polymerization or primer, followed by the transfer of the primer to a membrane-bound synthase. The latter catalyses the repetitive transfer of glucosyl units from an activated sugar donor to the acceptor-i.e. the primer or the elongating chain-until the polymerization stops. Most molecular events occurring during the biosynthesis of (1,3)-β-glucans and (1,3;1,6)-β-Glucans in Protozoans and Chromistans are not well understood. The process for which most biochemical data have been accumulated is the polymerization of linear (1,3)-β-glucan chains. The ability to synthesize β-glucans in vitro represents a very useful tool for assaying glucan synthases during enzyme purification. This is also useful in identifying the type of effectors that might influence enzyme activity in vivo and thus regulate the biosynthesis of the polysaccharides.

Place, publisher, year, edition, pages
Elsevier, 2009. 233-258 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-82663DOI: 10.1016/B978-0-12-373971-1.00007-8ISI: 000320860900008Scopus ID: 2-s2.0-84883919035ISBN: 978-012373971-1 OAI: oai:DiVA.org:kth-82663DiVA: diva2:498465
Note

QC 20120427

Available from: 2012-02-12 Created: 2012-02-12 Last updated: 2014-09-25Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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More styles
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Output format
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