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Turning of the receptor binding domains opens up the murine leukaemia virus Env for membrane fusion
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2008 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 27, no 20, 2799-2808 p.Article in journal (Refereed) Published
Abstract [en]

The activity of the membrane fusion protein Env of Moloney mouse leukaemia virus is controlled by isomerization of the disulphide that couples its transmembrane (TM) and surface (SU) subunits. We have arrested Env activation at a stage prior to isomerization by alkylating the active thiol in SU and compared the structure of isomerization-arrested Env with that of native Env. Env trimers of respective form were isolated from solubilized particles by sedimentation and their structures were reconstructed from electron microscopic images of both vitrified and negatively stained samples. We found that the protomeric unit of both trimers formed three protrusions, a top, middle and a lower one. The atomic structure of the receptor-binding domain of SU fitted into the upper protrusion. This was formed similar to a bent finger. Significantly, in native Env the tips of the fingers were directed against each other enclosing a cavity below, whereas they had turned outward in isomerization-arrested Env transforming the cavity into an open well. This might subsequently guide the fusion peptides in extended TM subunits into the target membrane.

Place, publisher, year, edition, pages
2008. Vol. 27, no 20, 2799-2808 p.
Keyword [en]
cryo-electron microscopy, Env trimers, image processing, isomerization-arrested state, receptor-binding domain
National Category
Natural Sciences
URN: urn:nbn:se:kth:diva-82946DOI: 10.1038/emboj.2008.187ISI: 000260501100016OAI: diva2:498609
QC 20120214Available from: 2012-02-12 Created: 2012-02-12 Last updated: 2012-05-08Bibliographically approved

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Hebert, HansKoeck, Philip J. B.
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