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Novel type II cell wall architecture in dichlobenil-habituated maize calluses
Universidad de León.
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2009 (English)In: Planta, ISSN 0032-0935, E-ISSN 1432-2048, Vol. 229, no 3, p. 617-631Article in journal (Refereed) Published
Abstract [en]

Growth of maize (Zea mays L.) callus-culture cells was inhibited using dichlobenil (2,6 dichlorobenzonitrile, DCB) concentrations > or =1 microM; I (50) value for the effect on inhibited fresh weight gain was 1.5 microM. By increasing the DCB concentration in the culture medium, DCB-habituated cells became 13 times more tolerant of the inhibitor (I (50): 20 microM). In comparison with non-habituated calluses, DCB-habituated calluses grew slower, were less friable and were formed by irregularly shaped cells surrounded by a thicker cell wall. By using an extensive array of techniques, changes in type II cell wall composition and structure associated with DCB habituation were studied. Walls from DCB-habituated cells showed a reduction of up to 75% in cellulose content, which was compensated for by a net increase in arabinoxylan content. Arabinoxylans also showed a reduction in their extractability and a marked increase in their relative molecular mass. DCB habituation also involved a shift from ferulate to coumarate-rich cells walls, and enrichment in cell wall esterified hydroxycinnamates and dehydroferulates. The content of polymers such as mixed-glucan, xyloglucan, mannans, pectins or proteins did not vary or was reduced. These results prove that the architecture of type II cell walls is able to compensate for deficiencies in cellulose content with a more extensive and phenolic cross-linked network of arabinoxylans, without necessitating beta-glucan or other polymer enhancement. As a consequence of this modified architecture, walls from DCB-habituated cells showed a reduction in their swelling capacity and an increase both in pore size and in resistance to polysaccharide hydrolytic enzymes.

Place, publisher, year, edition, pages
2009. Vol. 229, no 3, p. 617-631
Keyword [en]
Arabinoxylan, Callus culture, Cellulose, Dichlobenil, FTIR, Maize
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-66496DOI: 10.1007/s00425-008-0860-8ISI: 000262830800014PubMedID: 19048286OAI: oai:DiVA.org:kth-66496DiVA, id: diva2:499033
Note
QC 20120217Available from: 2012-02-13 Created: 2012-01-26 Last updated: 2017-12-07Bibliographically approved

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