High peroxidase activity and stable changes in the cell wall are related to dichlobenil tolerance
2009 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 166, no 12, 1229-1240 p.Article in journal (Refereed) Published
Suspension-cultured bean cells habituated to growth in a lethal concentration of dichlobenil were cultured for 3-5 years in a medium lacking the inhibitor in order to obtain long-term dehabituated cell lines. The growth parameters, cell morphology and ultrastructure of cells in the absence of dichlobenil reverted to that of non-habituated cells. The cellulose content and Fourier transform infrared (FTIR) spectra of crude cell walls from long-term dehabituated cells were also similar to those of non-habituated cells. However, long-term dehabituated cells showed three times more tolerance to dichlobenil than non-habituated cells. The incorporation of [(14)C]Glc into cellulose was reduced by 40% in dehabituated cells when compared with non-habituated cells. However, the addition of dichlobenil to dehabituated cells increased the incorporation of [(14)C]Glc into cellulose 3.3-fold with respect to that of non-habituated cells. Dehabituated cells showed a constitutively increased peroxidase activity when compared with non-habituated cells. Results reported here indicate that the habituation of bean cultured cells to dichlobenil relied partially on a stable change in the cellulose biosynthesis complex and is associated with high guaiacol peroxidase activity.
Place, publisher, year, edition, pages
2009. Vol. 166, no 12, 1229-1240 p.
[(14)C]glucose radiolabeling, Cell wall, Cellulose biosynthesis inhibitors, FTIR, Peroxidase
IdentifiersURN: urn:nbn:se:kth:diva-66494DOI: 10.1016/j.jplph.2009.01.014ISI: 000268818200001PubMedID: 19346029OAI: oai:DiVA.org:kth-66494DiVA: diva2:499034
QC 201202172012-02-132012-01-262012-02-17Bibliographically approved