The phenolic profile of maize primary cell wall changes in cellulose-deficient cell cultures
2010 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 71, no 14-15, 1684-1689 p.Article in journal (Refereed) Published
Cultured maize cells habituated to grow in the presence of the cellulose synthesis inhibitor dichlobenil (DCB) have a modified cell wall in which the amounts of cellulose are reduced and the amounts of arabinoxylan increased. This paper examines the contribution of cell wall-esterified hydroxycinnamates to the mechanism of DCB habituation. For this purpose, differences in the phenolic composition of DCB-habituated and non-habituated cell walls, throughout the cell culture cycle and the habituation process were characterized by HPLC. DCB habituation was accompanied by a net enrichment in cell wall phenolics irrespective of the cell culture phase. The amount of monomeric phenolics was 2-fold higher in habituated cell walls. Moreover, habituated cell walls were notably enriched in p-coumaric acid. Dehydrodimers were 5-6-fold enhanced as a result of DCB habituation and the steep increase in 8,5'-diferulic acid in habituated cell walls would suggest that this dehydrodimer plays a role in DCB habituation. In summary, the results obtained indicate that cell wall phenolics increased as a consequence of DCB habituation, and suggest that they would play a role in maintaining the functionality of a cellulose impoverished cell wall.
Place, publisher, year, edition, pages
2010. Vol. 71, no 14-15, 1684-1689 p.
Zea mays, Maize, Cell wall, DCB, Dichlobenil, Hydroxycinnamate, Ferulic acid, p-Coumaric acid, HPLC
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-66491DOI: 10.1016/j.phytochem.2010.06.013ISI: 000282453900009PubMedID: 20638694OAI: oai:DiVA.org:kth-66491DiVA: diva2:499039
QC 201202292012-02-132012-01-262012-02-29Bibliographically approved