Change search
ReferencesLink to record
Permanent link

Direct link
Changes in Cinnamic Acid Derivatives Associated with the Habituation of Maize Cells to Dichlobenil
Universidad de León.
Show others and affiliations
2011 (English)In: Molecular plant, ISSN 1752-9867, Vol. 4, no 5, 869-878 p.Article in journal (Refereed) Published
Abstract [en]

The habituation of cell cultures to cellulose biosynthesis inhibitors such as dichlobenil (DCB) represents a valuable tool to improve our knowledge of the mechanisms involved in plant cell wall structural plasticity. Maize cell lines habituated to lethal concentrations of DCB were able to grow through the acquisition of a modified cell wall in which cellulose was partially replaced by a more extensive network of arabinoxylans. The aim of this work was to investigate the phenolic metabolism of non-habituated and DCB-habituated maize cell cultures. Maize cell cultures were fed [(14)C]cinnamate and the fate of the radioactivity in different intra-protoplasmic and wall-localized fractions throughout the culture cycle was analyzed by autoradiography and scintillation counting. Non-habituated and habituated cultures did not markedly differ in their ability to uptake exogenous [(14)C]cinnamic acid. However, interesting differences were found in the radiolabeling of low- and high-M(r) metabolites. Habituated cultures displayed a higher number and amount of radiolabeled low-M(r) compounds, which could act as reserves later used for polysaccharide feruloylation. DCB-habituated cultures were highly enriched in esterified [(14)C]dehydrodiferulates and larger coupling products. In conclusion, an extensive and early cross-linking of hydroxycinnamates was observed in DCB-habituated cultures, probably strengthening their cellulose-deficient walls.

Place, publisher, year, edition, pages
2011. Vol. 4, no 5, 869-878 p.
Keyword [en]
Cell wall, Zea mays, maize, dichlobenil, DCB, dehydrodiferulate, ferulate, cinnamic acid
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-66488DOI: 10.1093/mp/ssr038ISI: 000295515500009PubMedID: 21571813OAI: diva2:499044
QC 20120229Available from: 2012-02-13 Created: 2012-01-26 Last updated: 2012-02-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Mélida, Hugo
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 17 hits
ReferencesLink to record
Permanent link

Direct link