Change search
ReferencesLink to record
Permanent link

Direct link
Development of a fed-batch process for the production of a recombinant protein X in CHO-GS system: Case study from the cell to reactor process ready for pilot scale cultivation
KTH, School of Biotechnology (BIO), Bioprocess Technology. (Cell Technology Group)ORCID iD: 0000-0002-5370-4621
Biovitrum/SOBI, Sweden.
Biovitrum/SOBI, Sweden.
Biovitrum/SOBI, Sweden.
Show others and affiliations
2010 (English)In: Cells and Culture: Proceedings of the 20th ESACT / [ed] Noll T, Springer Science+Business Media B.V., 2010, 723-725 p.Conference paper (Other academic)
Abstract [en]

A new cell line was created using CHO-GS system. The most promising clones were adapted to different base cultivation media leading to the selection of one medium. The fed-batch process development was performed in spinner, shake flask and bioreactor scale. It included the selection of a feed medium, the choice of the feed strategy and the optimisation of the glucose feeding. The process was then simplified by using a single feed including the feed medium and the glucose feed. Finally up-scaling parameters like aeration and CO2 stripping were studied in 3 L and 15 L bioreactors in preparation for pilot scale operation. This process proved to be robust, reproducible and suitable for large and commercial scale operation.

Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2010. 723-725 p.
Keyword [en]
National Category
Pharmaceutical Biotechnology
Research subject
SRA - Molecular Bioscience
URN: urn:nbn:se:kth:diva-86350DOI: 10.1007/978-90-481-3419-9_125ISBN: 978-90-481-3418-2OAI: diva2:500653
Proceedings of the 20th ESACT, Dresden, Germany, June 17-20, 2007
QC 20120420Available from: 2012-02-13 Created: 2012-02-13 Last updated: 2012-04-20Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Chotteau, Veronique
By organisation
Bioprocess Technology
Pharmaceutical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 81 hits
ReferencesLink to record
Permanent link

Direct link