Development of a fed-batch process for the production of a recombinant protein X in CHO-GS system: Case study from the cell to reactor process ready for pilot scale cultivation
2010 (English)In: Cells and Culture: Proceedings of the 20th ESACT / [ed] Noll T, Springer Science+Business Media B.V., 2010, 723-725 p.Conference paper (Other academic)
A new cell line was created using CHO-GS system. The most promising clones were adapted to different base cultivation media leading to the selection of one medium. The fed-batch process development was performed in spinner, shake flask and bioreactor scale. It included the selection of a feed medium, the choice of the feed strategy and the optimisation of the glucose feeding. The process was then simplified by using a single feed including the feed medium and the glucose feed. Finally up-scaling parameters like aeration and CO2 stripping were studied in 3 L and 15 L bioreactors in preparation for pilot scale operation. This process proved to be robust, reproducible and suitable for large and commercial scale operation.
Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2010. 723-725 p.
Research subject SRA - Molecular Bioscience
IdentifiersURN: urn:nbn:se:kth:diva-86350DOI: 10.1007/978-90-481-3419-9_125ISBN: 978-90-481-3418-2OAI: oai:DiVA.org:kth-86350DiVA: diva2:500653
Proceedings of the 20th ESACT, Dresden, Germany, June 17-20, 2007
QC 201204202012-02-132012-02-132012-04-20Bibliographically approved