Study of the effect of high pH and alkali addition in a cultivation of Chinese Hamster Ovary cell
2012 (English)In: Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT) / [ed] Jenkins, Nigel; Barron, Niall; Alves, Paula, Springer Science+Business Media B.V., 2012, 323-326 p.Conference paper (Refereed)
This work aimed at studying the impact of alkali addition in a Chinese Hamster Ovary cell culture. Two phenomena were studied, the kinetic rate of direct cell death in presence of high pH and the effect of transitory single contact of high pH on cell viability and growth. Contact with pH 11 or 10 did not provoke immediate cell lysis. The cells survived several minutes to such conditions. Contact with pH 11 during 2 minutes, with pH 10 during 5 minutes, with pH 9 during 5 minutes or 10 minutes did not affect the viability. In these conditions, the growth was not affected except after 5 minutes contact at pH 10 or 10 minutes contact at pH 9 for which the growth was slowed down the first day only. As expected, NaOH addition affected the cells more than Na2CO3 addition. This was due to a higher pH but could be even observed at the same pH (10).
Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2012. 323-326 p.
pH, alkali, Chinese Hamster Ovary cell
Research subject SRA - Molecular Bioscience
IdentifiersURN: urn:nbn:se:kth:diva-86381DOI: 10.1007/978-94-007-0884-6_49ISBN: 978-94-007-0883-9OAI: oai:DiVA.org:kth-86381DiVA: diva2:500679
21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009
QC 201212182012-02-132012-02-132012-12-18Bibliographically approved