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Study of the effect of high pH and alkali addition in a cultivation of Chinese Hamster Ovary cell
KTH, School of Biotechnology (BIO), Bioprocess Technology (closed 20130101). (Cell Technology Group)ORCID iD: 0000-0002-5370-4621
KTH, School of Biotechnology (BIO), Bioprocess Technology (closed 20130101). (Cell Technology Group)
2012 (English)In: Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT) / [ed] Jenkins, Nigel; Barron, Niall; Alves, Paula, Springer Science+Business Media B.V., 2012, 323-326 p.Conference paper, Published paper (Refereed)
Abstract [en]

This work aimed at studying the impact of alkali addition in a Chinese Hamster Ovary cell culture. Two phenomena were studied, the kinetic rate of direct cell death in presence of high pH and the effect of transitory single contact of high pH on cell viability and growth. Contact with pH 11 or 10 did not provoke immediate cell lysis. The cells survived several minutes to such conditions. Contact with pH 11 during 2 minutes, with pH 10 during 5 minutes, with pH 9 during 5 minutes or 10 minutes did not affect the viability. In these conditions, the growth was not affected except after 5 minutes contact at pH 10 or 10 minutes contact at pH 9 for which the growth was slowed down the first day only. As expected, NaOH addition affected the cells more than Na2CO3 addition. This was due to a higher pH but could be even observed at the same pH (10).

Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2012. 323-326 p.
Keyword [en]
pH, alkali, Chinese Hamster Ovary cell
National Category
Pharmaceutical Biotechnology
Research subject
SRA - Molecular Bioscience
Identifiers
URN: urn:nbn:se:kth:diva-86381DOI: 10.1007/978-94-007-0884-6_49ISBN: 978-94-007-0883-9 (print)OAI: oai:DiVA.org:kth-86381DiVA: diva2:500679
Conference
21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009
Note

QC 20121218

Available from: 2012-02-13 Created: 2012-02-13 Last updated: 2016-12-22Bibliographically approved

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Chotteau, Veronique

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CiteExportLink to record
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