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Study of a recombinant CHO cell line producing a monoclonal antibody by ATF or TFF external filter perfusion in a WAVE Bioreactor™
KTH, School of Biotechnology (BIO), Bioprocess Technology.
KTH, School of Biotechnology (BIO), Bioprocess Technology.
KTH, School of Biotechnology (BIO), Bioprocess Technology.ORCID iD: 0000-0002-0841-8845
GE Healthcare, Uppsala, Sweden.
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2011 (English)In: BMC Proceedings, 2011, Volume 5, Supplement 8, P105, BioMed Central, 2011, 105- p.Conference paper, Published paper (Refereed)
Abstract [en]

Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. Moreover, perfusion is optimal when the product of interest is unstable or if the product yield is low. On the other hand, disadvantages are for example technical challenges originating from non-robust cell separation devices as well as sterility concerns from the more complex set-up needed.

In the present work, the use of a WAVE Bioreactor™ system 20/50 in perfusion mode with10 L disposable Cellbag™ bioreactors customized with two dip tubes in combination with disposable hollow fiber filters as external cell separating devices were investigated. A comparison between Alternating Tangential Flow (ATF) and Tangential Flow Filtration (TFF) was performed using a recombinant CHO cell line producing a monoclonal antibody (mAb) as a model system. 

Place, publisher, year, edition, pages
BioMed Central, 2011. 105- p.
Keyword [en]
perfusion, ATF, TFF, hollow fiber, cell separation, Chinese Hamster Ovary, wave bioreactor, high cell density
National Category
Pharmaceutical Biotechnology
Research subject
SRA - Molecular Bioscience
Identifiers
URN: urn:nbn:se:kth:diva-86387DOI: 10.1186/1753-6561-5-S8-P105OAI: oai:DiVA.org:kth-86387DiVA: diva2:500688
Conference
22d ESACT (European Society for Animal Cell Technology), May 15-18, 2011, Vienna, Austria
Note
QC 20120214Available from: 2012-02-14 Created: 2012-02-13 Last updated: 2012-03-22Bibliographically approved

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Zhang, YeChotteau, Veronique

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Clincke, Marie-FrancoiseMölleryd, CarinZhang, YeChotteau, Veronique
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CiteExportLink to record
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