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Perfusion of an IgG producing CHO cell line at very high cell density by ATF or by TFF in WAVE Bioreactor™
KTH, School of Biotechnology (BIO), Bioprocess Technology. (Cell Technology Group)
KTH, School of Biotechnology (BIO), Bioprocess Technology. (Cell Technology Group)
KTH, School of Biotechnology (BIO), Bioprocess Technology. (Cell Technology Group)
KTH, School of Biotechnology (BIO), Bioprocess Technology. (Cell Technology Group)ORCID iD: 0000-0002-0841-8845
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2011 (English)Conference paper, Published paper (Other academic)
Abstract [en]

Perfusion of an IgG producing CHO cell line was performed in a WAVE Bioreactor™ using either Alternating Tangential Flow or Tangential Flow Filtration. The properties and performances obtained in this bioreactor with both filtration systems were studied.

  • Very high cell densities were achieved and could be stably maintained at high viability indicating of a healthy process suitable for instance for efficient cell banking.

Cell density could be significantly further increased showing the capacity of the system set-up.

Place, publisher, year, edition, pages
2011.
Keyword [en]
perfusion, hollow fiber filter, perfusion, ATF, TFF, CHO cells
National Category
Medical Biotechnology
Research subject
SRA - Molecular Bioscience
Identifiers
URN: urn:nbn:se:kth:diva-87442OAI: oai:DiVA.org:kth-87442DiVA: diva2:501653
Conference
HansonWade Conference Optimizing Cell Line Performance, July, 26-28, 2011, London, UK
Note
Invited speaker at the Hanson Wade Conference Optimizing Cell Line Performance, July, 26-28, 2011, London, UK. QC 20130319Available from: 2012-02-14 Created: 2012-02-14 Last updated: 2012-03-19Bibliographically approved

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http://cell-linesummit.com/

Authority records BETA

Zhang, YeChotteau, Veronique

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Clincke, Marie-FrancoiseMölleryd, CarinSamani, Puneeth K.Zhang, YeLindskog, EvaChotteau, Veronique
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Citation style
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  • harvard1
  • ieee
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  • vancouver
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Language
  • de-DE
  • en-GB
  • en-US
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  • nn-NB
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  • Other locale
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Output format
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  • asciidoc
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