Mastering Oxygen and Carbon Dioxide in Cell Culture
2010 (English)Other (Other academic)
Mammalian cells need adequate aeration for the cellular respiration and simultaneously produce carbon dioxide. Aeration in pilot and large scale cultivation is operated by blowing oxygen or oxygen enriched air in the culture, i.e. sparging. The gas bubbles reaching the surface generate foam formation, which is well known to be damageable for the cells. Therefore, it can be preferable to reduce the bubble sparging while maintaining the oxygen concentration in the culture. Carbon dioxide produced by the cells can accumulate in the culture in case its removal rate from the liquid phase is not fast enough. This provokes an acidification of the medium, which has to be compensated by alkali addition. High levels of carbon dioxide and high levels of alkali can be damageable for the process performances. A common way to actively remove the carbon dioxide from the liquid phase is to benefit from the bubbles moving to the liquid surface. Obviously, large scale cultivation can present a dilemma in which reduced sparging is favoured for the aeration strategy in order to avoid cell damage by excessive foaming while increased sparging provide reduced accumulation of carbon dioxide. Typical approaches used in the biopharmaceutical industry for aeration and carbon dioxide removal will be reviewed in the presentation.
Place, publisher, year, edition, pages
oxygenation, carbon dioxide, animal cell culture
Research subject SRA - Molecular Bioscience
IdentifiersURN: urn:nbn:se:kth:diva-87469OAI: oai:DiVA.org:kth-87469DiVA: diva2:501686
Invited speaker at the Workshop Cell Culture Economy, Technology and Solutions, CELS, CEFFORT, Nov 11, 2010, Stockholm, Sweden2012-02-142012-02-142012-02-23Bibliographically approved