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Fed-batch or perfusion for the production of biopharmaceuticals by animal cell cultivation?
KTH, School of Biotechnology (BIO), Bioprocess Technology. (Cell Technology Group)ORCID iD: 0000-0002-5370-4621
2009 (English)Other (Other academic)
Abstract [en]

Fed-batch or perfusion modes are today’s options for the cultivation process development of new candidates drugs. In the cases of unstable proteins, perfusion is the obligatory choice. Otherwise, this choice is dictated by the need of simplicity, the existing technical know-how in the company and the available equipment (e.g. bioreactor size, perfusion device, etc.). The higher risk of contamination and higher technical challenge of perfusion processes lead often to the selection of fed-batch for commercial processes. This is reinforced today in the case of antibody production in established biotech companies by the use of generic fed-batch processes with high yield production and low accumulation of toxic by-products (e.g. lactate, ammonia). However perfusion can be a valuable option due to the usage of smaller bioreactors, today’s availability of scalable low shear force perfusion system (e.g. ATF) and potentially the lower requirement of process development for perfusion. Perfusion can therefore be an attractive choice for instance for the production of glycoproteins, which are not antibody, and/or for small companies, which do not have generic process.

Place, publisher, year, edition, pages
Keyword [en]
perfusion, fed-batch, animal cell culture
National Category
Medical Biotechnology
Research subject
SRA - Molecular Bioscience
URN: urn:nbn:se:kth:diva-87478OAI: diva2:501692
Invited speaker at the workshop Securing Quality in Bioanalysis and Production, CELS, CEFFORT, Nov 25, 2009, Stockholm, Sweden. QC 20120214Available from: 2012-02-14 Created: 2012-02-14 Last updated: 2012-02-14Bibliographically approved

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