Mutated immunoglobulin-binding protein
2002 (English)Patent (Other (popular science, discussion, etc.))
The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.
Place, publisher, year, edition, pages
IdentifiersURN: urn:nbn:se:kth:diva-87882OAI: oai:DiVA.org:kth-87882DiVA: diva2:501985
EP 1485407-B1 (2009-05-13)
JP 4391830-B2 (2009-12-24); AU 2003217119-B2 (2010-02-18); US 7834158-B2 (2010-11-16); CN 1642976-B (2012-02-22); US 8198404-B2 (2012-06-12); EP 1485407-B2 (2012-09-26); US 8354510-B2 (2013-01-15); CN 102532284 B (2014-04-16)2012-02-142012-02-142015-05-12Bibliographically approved