Change search
ReferencesLink to record
Permanent link

Direct link
Genome-wide transcriptome analysis of 150 cell samples
Harvard Univ, Sch Med, Boston ; Massachusetts Gen Hosp, Shriners Hosp Children.
Show others and affiliations
2009 (English)In: Integrative Biology, ISSN 1757-9694, Vol. 1, no 1, 99-107 p.Article in journal (Refereed) Published
Abstract [en]

 major challenge in molecular biology is interrogating the human transcriptome on a genome wide scale when only a limited amount of biological sample is available for analysis. Current methodologies using microarray technologies for simultaneously monitoring mRNA transcription levels, require nanogram amounts of total RNA. To overcome the sample size limitation of Current technologies, we have developed a method to probe the global gene expression ill biological samples as small as 150 cells, or the equivalent of approximately 300 pg total RNA. The new method employs microfluidic devices for the purification of total RNA from mammalian cells and ultra-sensitive whole transcriptome amplification techniques. We verified that the RNA integrity is preserved through the isolation process, accomplished highly reproducible whole transcriptome analysis, and established high correlation between repeated isolations of 150 cells and the same cell culture sample. We validated the technology by demonstrating that the combined microfluidic and amplification protocol is capable of identifying biological pathways perturbed by Stimulation, which are consistent with the information recognized in bulk-isolated samples.

Place, publisher, year, edition, pages
2009. Vol. 1, no 1, 99-107 p.
National Category
Natural Sciences
URN: urn:nbn:se:kth:diva-89923DOI: 10.1039/b814329cISI: 000266978200011OAI: diva2:503990
QC 20120219Available from: 2012-02-17 Created: 2012-02-17 Last updated: 2012-02-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Russom, Aman
In the same journal
Integrative Biology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 12 hits
ReferencesLink to record
Permanent link

Direct link