Change search
ReferencesLink to record
Permanent link

Direct link
Chemical gradient-mediated melting curve analysis for genotyping of SNPs
Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston.
2009 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 30, no 14, 2536-2543 p.Article in journal (Refereed) Published
Abstract [en]

This report describes a microfluidic solid-phase chemical gradient-mediated melting curve analysis method for SNP analysis. The method is based on allele-specific denaturation to discriminate mismatched (MM) from perfectly matched (PM) DNA duplexes upon exposure to linear chemical gradient. PM and MM DNA duplexes conjugated on beads are captured in a microfluidic gradient generator device designed with dams, keeping the beads trapped perpendicular to a gradient generating channel. Two denaturants, formamide and urea, were tested for their ability to destabilize the DNA duplex by competing with Watson-Crick pairing. Upon exposure to the chemical gradient, rapid denaturing profile was monitored in real time using fluorescence microscopy. The results show that the two duplexes exhibit different kinetics of denaturation profiles, enabling discrimination of MM from PM DNA duplexes to score SNP.

Place, publisher, year, edition, pages
2009. Vol. 30, no 14, 2536-2543 p.
Keyword [en]
Chemical gradient, Formamide, Melting curve analysis, SNP, Urea
National Category
Natural Sciences
URN: urn:nbn:se:kth:diva-89922DOI: 10.1002/elps.200800729ISI: 000269041500017OAI: diva2:503994
QC 20120312Available from: 2012-02-17 Created: 2012-02-17 Last updated: 2012-03-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Russom, Aman
In the same journal
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 24 hits
ReferencesLink to record
Permanent link

Direct link