Biomolecular association – biolubrication perspective: Association between hyaluronan and phospholipids
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Biomolekylers association-biolubricerings perspektiv: Association mellan hyaluronan och fosfolipider (Swedish)
Dipalmitoylphosphatidylcholine (DPPC) vesicles were prepared by sonication and their size in sodium chloride solutions ([NaCl] = 0.116 M) containing different amount of calcium ions (0, 1, 2, 5, 10 mM) were studied by Dynamic Light Scattering (DLS). The time dependence of the particle size in various solutions was also tested. The data showed that the hydrodynamic diameter of DPPC vesicles was not affected by the Ca2+ concentration; however, the stability of DPPC vesicles was improved with the presence of Ca2+. Besides, when the temperature was above the phase transition temperature (41.5°C), the DPPC vesicles in dispersions with more than 2 mM CaCl2 remained stable for at least 2 weeks. Zeta potential of vesicles in aqueous solutions was tested by Zetasizer. The result showed that the stability of DPPC vesicles increased with increasing Ca2+ concentration with the evidence of increasing zeta potential due to the binding of Ca2+ onto vesicle bilayers. The association between zwitterionic DPPC vesicles and anionic polyelectrolyte hyaluronan (HA) was also studied by testing the hydrodynamic diameter and electrophoretic mobility change after the addition of HA. DLS results showed that the hydrodynamic diameter of DPPC vesicles increased in the presence of HA. In addition, after several days’ incubation at 55°C precipitation appeared in the DPPC-HA mixture solution. Furthermore, electrophoretic mobility of DPPC vesicles decreased after the addition of polyelectrolyte. The combined results demonstrated that the association between DPPC and HA occurred.
Place, publisher, year, edition, pages
2011. , 44 p.
Biolubrication, molecular association, phospholipid, polyelectrolyte, hyaluronan
IdentifiersURN: urn:nbn:se:kth:diva-90386OAI: oai:DiVA.org:kth-90386DiVA: diva2:505320
Subject / course
Chemical Science and Engineering
Degree of Master
UppsokPhysics, Chemistry, Mathematics
Claesson, Per, Professor