Photogenerated lectin sensors produced by thiol-ene/yne photo-click chemistry in aqueous solution
2012 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 34, no 1, 51-56 p.Article in journal (Refereed) Published
The photoinitiated radical reactions between thiols and alkenes/alkynes (thiol-ene and thiol-yne chemistry) have been applied to a functionalization methodology to produce carbohydrate-presenting surfaces for analyses of biomolecular interactions. Polymer-coated quartz surfaces were functionalized with alkenes or alkynes in a straightforward photochemical procedure utilizing perfluorophenylazide (PFPA) chemistry. The alkene/alkyne surfaces were subsequently allowed to react with carbohydrate thiols in water under UV-irradiation. The reaction can be carried out in a drop of water directly on the surface without photoinitiator, and any disulfide side products were easily washed away after the functionalization process. The resulting carbohydrate-presenting surfaces were evaluated in real-time studies of protein-carbohydrate interactions using a quartz crystal microbalance (QCM) flow-through system with recurring injections of selected lectins, with intermediate regeneration steps using low pH buffer. The resulting methodology proved fast, efficient and scalable to high-throughput analysis formats, and the produced surfaces showed significant protein binding with expected selectivities of the lectins used in the study.
Place, publisher, year, edition, pages
2012. Vol. 34, no 1, 51-56 p.
Photochemistry, Click chemistry, Thiol-ene/-yne, Carbohydrates, Lectins, Quartz crystal microbalance
IdentifiersURN: urn:nbn:se:kth:diva-90846DOI: 10.1016/j.bios.2012.01.001ISI: 000302886500007ScopusID: 2-s2.0-84858216975OAI: oai:DiVA.org:kth-90846DiVA: diva2:507542
FunderSwedish Research Council, 621-2007-4709
QC 201205252012-03-052012-03-012012-05-25Bibliographically approved