Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Method of sequencing DNA
1996 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

The present invention provides a method of identifying a base at a target position in a single-stranded sample DNA sequence wherein an extension primer, which hybridizes to the sample DNA immediately adjacent to the target position, is provided and the sample DNA and extension primer are subjected to a polymerization reaction in the presence of a deoxynucleotide or dideoxynucleotide, whereby the deoxynucleotide or dideoxynucleotide will only become incorporated and release pyrophosphate if it is complementary to the base in the target position. Release of pyrophosphate is detected enzymatically and pyrophosphate detection enzyme(s) are included in the polymerization step.

Place, publisher, year, edition, pages
1996.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-91210OAI: oai:DiVA.org:kth-91210DiVA: diva2:508826
Patent
US 6210891-B1 (2001-04-03)
Note

EP 0932700-B1 (2001-08-29); JP 3510272-B2 (2004-03-22)

Available from: 2012-03-09 Created: 2012-03-09 Last updated: 2013-08-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Espacenet

Authority records BETA

Uhlén, Mathias

Search in DiVA

By author/editor
Uhlén, Mathias
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 26 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf