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Signaling pathways involved in the regulation of TNF alpha-induced toll-like receptor 2 expression in human gingival fibroblasts
KTH, School of Biotechnology (BIO), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-4313-1601
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2012 (English)In: Cytokine, ISSN 1043-4666, E-ISSN 1096-0023, Vol. 57, no 3, 406-416 p.Article in journal (Refereed) Published
Abstract [en]

Periodontitis is a chronic inflammatory disease characterized by a host inflammatory response against bacteria that leads to destruction of the supporting structures of the teeth. Bacterial components of pathogens in the periodontal pocket are recognized by toll-like receptors (TLRs) that trigger an inflammatory response. In this study, we investigated the effects of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF alpha) on TLR2 expression in human gingival fibroblasts. In addition, we examined the signaling pathways involved in the regulation of TNF alpha-induced TLR2 expression. Our results showed that TNF alpha increased TLR2 mRNA and protein expression. Microarray analysis and the inhibition of specific signaling pathways demonstrated that c-Jun N-terminal kinases (JNK) and nuclear factor kappa B (NF-kappa B) were involved in the regulation of INF alpha-induced TLR2 expression in gingival fibroblasts. Furthermore, the prostaglandin E-2 (PGE(2)) regulatory enzyme cytosolic phospholipase A(2) (cPLA(2)) and the anti-inflammatory prostaglandin 15-deoxy-Delta(12.14)-prostaglandin J(2) (15d-PGJ(2)), were found to regulate TLR2 mRNA expression stimulated by TNF alpha. Our findings suggest that these pathways and mediators, through the regulation of TLR2 expression in gingival fibroblasts, may be involved in the pathogenesis of periodontitis. The study provides new insights into the molecular mechanisms underlying the regulation of TLR2, implicated in the chronic inflammatory disease periodontitis.

Place, publisher, year, edition, pages
2012. Vol. 57, no 3, 406-416 p.
Keyword [en]
TLR2, TNF alpha, Gingival fibroblasts, Microarray, Prostaglandins
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-92049DOI: 10.1016/j.cyto.2011.12.008ISI: 000300868000014Scopus ID: 2-s2.0-84856370445OAI: oai:DiVA.org:kth-92049DiVA: diva2:512239
Funder
Swedish Research Council, 73XD-15005
Note
QC 20120327Available from: 2012-03-27 Created: 2012-03-26 Last updated: 2017-12-07Bibliographically approved

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