Change search
ReferencesLink to record
Permanent link

Direct link
p- and m-Cresols emitted from estrous urine are reliable volatile chemical markers of ovulation in mares
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry. (Ecological Chemistry Group)
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry. (Ecological Chemistry Group)
2012 (English)In: Animal Reproduction Science, ISSN 0378-4320, E-ISSN 1873-2232, Vol. 130, no 1-2, 51-56 p.Article in journal (Refereed) Published
Abstract [en]

Urine samples from 14 mares, belonging to five breeds, were collected at estrus and diestrus to search for estrous specific volatile compounds which could be used to determine ovulation time. Around 150 volatiles were collected from urine head-space samples by solid phase micro extraction technique, and analyses were conducted by gas chromatography-mass spectrometry methods. Comparison of chromatographic profiles of volatile substances revealed that concentrations of m- and p-cresols were significantly greater during estrus while diethylphthalate was more abundant at diestrus. Monitoring of m- and p-cresols during the period of estrus and a few days before and after estrus revealed irregular changes in amounts of cresols until 3-4 days before ovulation when the concentration of the compounds began to increase with peaks 1 day before ovulation. On the day when ovulation occurred, amounts of the metabolites decreased sharply, almost to basal concentrations, and remained at these concentrations for 6 days - when sampling was finished. In four of the mares changes in the concentration of diethylphthalate were less pronounced and more temporally variable compared with those of cresols. Based on reproducible temporal changes in concentrations of m- and p-cresols, with respect to the time of ovulation, a noninvasive test to determine a precise insemination time could occur. This would save time, reduce costs and simplify the procedure.

Place, publisher, year, edition, pages
2012. Vol. 130, no 1-2, 51-56 p.
Keyword [en]
Phenolic, Equus cabalus, Horse, Estrus, Headspace, SPME
National Category
Organic Chemistry
URN: urn:nbn:se:kth:diva-92368DOI: 10.1016/j.anireprosci.2011.12.008ISI: 000301275400007ScopusID: 2-s2.0-84856793449OAI: diva2:516083
QC 20120417Available from: 2012-04-17 Created: 2012-04-02 Last updated: 2012-04-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Borg Karlsson, Anna-Karin
By organisation
Organic Chemistry
In the same journal
Animal Reproduction Science
Organic Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 25 hits
ReferencesLink to record
Permanent link

Direct link