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Microsomal Glutathione Transferase 1 Protects Against Toxicity Induced by Silica Nanoparticles but Not by Zinc Oxide Nanoparticles
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2012 (English)In: ACS Nano, ISSN 1936-0851, Vol. 6, no 3, 1925-1938 p.Article in journal (Refereed) Published
Abstract [en]

Microsomal glutathione transferase 1 (MGST1) is an antioxidant enzyme located predominantly in the mitochondrial er membrane and endoplasmk reticulum and has been shown to protect cells from lipid peroxidation induced by a variety of cytostatic drugs and pro-oxidant stimuli. We hypothesized that MGST1 may also protect against nanomaterial-induced cytotoxicity through a specific effect on lipid peroxidation. We evaluated the induction of cytotoxicity and oxidative stress by TiO2, CeO2, SiO2, and ZnO in the human MCF-7 cell line with or without overexpression of MGST1. SiO2 and ZnO nanoparticles caused dose- and time-dependent toxicity, whereas no obvious cytotoxic effects were induced by nanoparticles of TiO2 and CeO2. We also noted pronounced cytotoxicity for three out of four additional SiO2 nanoparticles tested. Overexpression of MGST1 reversed the cytotoxicity of the main SiO2 nanoparticles tested and for one of the supplementary SiO2 nanoparticles but did not protect cells against ZnO-induced cytotoxic effects. The data point toward a role of lipid peroxidation In SiO2 nanoparticle-induced cell death. For ZnO nanoparticles, rapid dissolution was observed, and the subsequent interaction of Zn2+ with cellular targets is likely to contribute to the cytotoxic effects. A direct inhibition of MGST1 by Zn2+ could provide a possible explanation for the lack of protection against ZnO nanoparticles in this model. Our data also showed that SiO2 nanoparticle-induced cytotoxicity is mitigated in the presence of serum, potentially through masking of reactive surface groups by serum proteins, whereas ZnO nanoparticles were cytotoxic both In the presence and in the absence of serum.

Place, publisher, year, edition, pages
2012. Vol. 6, no 3, 1925-1938 p.
Keyword [en]
engineered nanoparticles, oxidative stress, lipid peroxidation, microsomal glutathione transferase 1
National Category
Other Materials Engineering
URN: urn:nbn:se:kth:diva-93650DOI: 10.1021/nn2021056ISI: 000301945900006ScopusID: 2-s2.0-84859147188OAI: diva2:517467
EU, European Research Council, NMP-SL-2008-214281Swedish Research Council
QC 20120423Available from: 2012-04-23 Created: 2012-04-23 Last updated: 2016-01-11Bibliographically approved
In thesis
1. Application of Nanomaterials for the Removal of Hexavalent Chromium and their Biological Implications
Open this publication in new window or tab >>Application of Nanomaterials for the Removal of Hexavalent Chromium and their Biological Implications
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The International Agency for Research on Cancer (IARC) stated that chromium in the form of Cr(VI) has been deemed to be a class-A human carcinogen. It has been a major contaminant associated with wastewater. Moreover, the existence of heavy metals in aquatic systems is a critical concern for the environment as well as industries that manufacture or consume these particular elements. In order to remove these particular toxic metals, several well-known conventional methods including ion-exchange, filtration and adsorption are used. Amongst these methods, adsorption offers significant advantages such as the low-cost materials, ease of operation and efficiency in comparison to the other conventional methods.

The aim of this work was to develop nanomaterials (particles and fibers) to address some critical issues for the treatment of heavy metals, especially chromium in aqueous systems. Furthermore, the use of nanomaterials and how they relate to nanoscale operations at the biological level has generated considerable concerns in spite of their novel properties.

The first part of this thesis deals with the synthesis and characterizations of Fe3O4, magnetite, as nanoparticles which were further coated with surfactants bis(2,4,4-trimethylpentyl)dithiophosphinic acid, Cyanex-301, and 3-Mercaptopropionic acid with the active compound being the thiol (SH) groups, that will suffice as a viable material for Cr(VI) removal from aqueous solutions. The proposed mechanism was the complexation between the thiol group on Cyanex-301 and 3-Mercaptopropionic acid, respectively. The effect of different parameters on the adsorption including contact time, initial and final Cr(VI) ion concentration and solution pH was investigated.

The second part of this thesis encompassed the fabrication of flexible nanocomposite materials, with a large surface area and architecture for the removal of Cr(VI) in batch and continuous flow mode. A technique known as electrospinning was used to produce the nanofibers. The flexible yet functional materials architecture has been achieved by growing ZnO nanorod arrays through chemical bath deposition on synthesized electrospun poly-L-lactide nanofibers. Moreover, polyacrylonitrile nanofibers (PAN) were synthesized and adapted by the addition of hydroxylamine hydrochloride to produce amidoxime polyacrylonitrile nanofibers (A-PAN). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to identify the morphologies and particle sizes whereas Fourier-Transform Infrared spectroscopy (FT-IR) was used to identify either the presence or absence of functional groups for the formation of PAN and A-PAN nanofibers. The optimization of functionalized nanoadsorbents to adsorb Cr(VI) was also carried out to investigate the effect of experimental parameters: contact time, solution pH, initial, final and other metal ion concentration. Commercially manufactured pristine engineered (TiO2, ZnO and SiO2) nanoparticles and lab-made functionalized (Fe3O4 and CeO2) nanoparticles were studied while the powders were suspended in appropriate media by Dynamic Light Scattering (DLS) to identify their cytotoxicity effects.

Place, publisher, year, edition, pages
KTH: KTH Royal Institute of Technology, 2016. 76 p.
TRITA-CHE-Report, ISSN 1654-1081 ; 2016:4
Nanomaterials, Chromium, Biology
National Category
Engineering and Technology
Research subject
Chemical Engineering
urn:nbn:se:kth:diva-179871 (URN)978-91-7595-813-2 (ISBN)
Public defence
2016-01-29, sal D2, Lindstedisvägen 5, KTH, Stockholm, 10:00 (English)

QC 20160111

Available from: 2016-01-11 Created: 2016-01-04 Last updated: 2016-01-11Bibliographically approved

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