Comparison and Functionalization Study of Microemulsion-Prepared Magnetic Iron Oxide Nanoparticles
2012 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 28, no 22, 8479-8485 p.Article in journal (Refereed) Published
Magnetic iron oxide nanoparticles (MION) for protein binding and separation were obtained from water-in-oil (w/o) and oil-in-water (o/w) microemulsions. Characterization of the prepared nanoparticles have been performed by TEM, XRD, SQUID magnetometry, and BET. Microemulsion-prepared magnetic iron oxide nanoparticles (ME-MION) with sizes ranging from 2 to 10 rim were obtained. Study on the magnetic properties at 300 K shows a large increase of the magnetization similar to 35 emu/g for w/o-ME-MION with superparamagnetic behavior and nanoscale dimensions in comparison with o/w-ME-MION (10 emu/g) due to larger particle size and anisotropic property. Moringa oleifera coagulation protein (MOCP) bound w/o- and o/w-ME-MION showed an enhanced performance in terms of coagulation activity. A significant interaction between the magnetic nanoparticles and the protein can be described by changes in fluorescence emission spectra. Adsorbed protein from MOCP is still retaining its functionality even after binding to the nanoparticles, thus implying the extension of this technique for various applications.
Place, publisher, year, edition, pages
2012. Vol. 28, no 22, 8479-8485 p.
Anisotropic property, Coagulation proteins, Fluorescence emission spectra, Functionalizations, Larger particle sizes, Magnetic iron oxide nanoparticles, Magnetic nanoparticles, Moringa oleifera, Nanoscale dimensions, Oil-in-water, Protein binding, SQUID magnetometry, Superparamagnetic behavior, Water-in-oil, XRD, Biochemistry, Coagulation, Emission spectroscopy, Magnetic properties, Metal nanoparticles, Proteins, Superparamagnetism
IdentifiersURN: urn:nbn:se:kth:diva-94164DOI: 10.1021/la300599qISI: 000304783300024ScopusID: 2-s2.0-84861871386OAI: oai:DiVA.org:kth-94164DiVA: diva2:525681
QC 20120703. Updated from in press to published.2012-05-092012-05-092012-07-03Bibliographically approved