Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Narrow genetic basis for the Australian dingo confirmed through analysis of paternal ancestry
KTH, School of Biotechnology (BIO), Gene Technology.
KTH, School of Biotechnology (BIO), Gene Technology.
KTH, School of Biotechnology (BIO), Gene Technology.
Show others and affiliations
2012 (English)In: Genetica, ISSN 0016-6707, E-ISSN 1573-6857, Vol. 140, no 1-3, 65-73 p.Article in journal (Refereed) Published
Abstract [en]

The dingo (Canis lupus dingo) is an iconic animal in the native culture of Australia, but archaeological and molecular records indicate a relatively recent history on the continent. Studies of mitochondrial DNA (mtDNA) imply that the current dingo population was founded by a small population of already tamed dogs from Southeast Asia. However, the maternal genetic data might give a unilateral picture, and the gene pool has yet to be screened for paternal ancestry. We sequenced 14,437 bp of the Y-chromosome (Y-chr) from two dingoes and one New Guinea Singing Dog (NGSD). This positioned dingo and NGSD within the domestic dog Y-chr phylogeny, and produced one haplotype not detected before. With this data, we characterized 47 male dingoes in 30 Y-chr single-nucleotide polymorphism sites using protease-mediated allele-specific extension technology. Only two haplotypes, H3 and H60, were found among the dingoes, at frequencies of 68.1 and 31.9 %, respectively, compared to 27 haplotypes previously established in the domestic dog. While H3 is common among Southeast Asian dogs, H60 was specifically found in dingoes and the NGSD, but was related to Southeast Asian dog Y-chr haplotypes. H3 and H60 were observed exclusively in the western and eastern parts of Australia, respectively, but had a common range in Southeast. Thus, the Y-chr diversity was very low, similar to previous observations for d-loop mtDNA. Overall genetic evidence suggests a very restricted introduction of the first dingoes into Australia, possibly from New Guinea. This study further confirms the dingo as an isolated feral dog.

Place, publisher, year, edition, pages
2012. Vol. 140, no 1-3, 65-73 p.
Keyword [en]
Dingo, Canis familiaris, New Guinea Singing Dog, Y-chromosome, Single Nucleotide Polymorphism (SNP), Protease-mediated Allele-specific Extension (PrASE), Short Interspersed Element (SINE)
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-94527DOI: 10.1007/s10709-012-9658-5ISI: 000305886800006Scopus ID: 2-s2.0-84863336209OAI: oai:DiVA.org:kth-94527DiVA: diva2:526004
Funder
Swedish Research CouncilKnut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20120730

Available from: 2012-05-10 Created: 2012-05-09 Last updated: 2017-12-07Bibliographically approved
In thesis
1. Analysis of the origin and spread of the domestic dog using Y-chromosome DNA and mtDNA sequence data
Open this publication in new window or tab >>Analysis of the origin and spread of the domestic dog using Y-chromosome DNA and mtDNA sequence data
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The domestic dog was probably the first domesticated animal, and the only one to spread to all continents in ancient times. The dog is one of the most phenotypically diverse animals, a result of human selection throughout dog history. Studies of the genetic origins and early spread of domestic dogs is important to gather information about biological and cultural mechanisms behind domestication but also to investigate early human history. The step from a hunter and gatherer lifestyle to farming is one of the most important steps in human history. In this thesis I will present work aimed at understanding both domestic dog origins and dispersal. In order to be able to investigate dog origins based on a second haploid chromosome we identified 14,437 bp of Y-chromosomal DNA sequence. Based on this we show that dogs in Asia south of Yangtze River (ASY) has the highest genetic diversity and was founded from a large number of wolf founders confirming earlier mtDNA results. Early dog dispersal is tightly coupled to human history with the dog brought along as a cultural item. We have for the first time investigated the dog dispersal into Polynesia and Australia and our data can be used as evidence for a more complex settlement of Polynesia than earlier indicated from archaeological and linguistic studies. Analysis of Y-chromosome SNPs in Australian dingoes confirms earlier mtDNA genetic studies that the dingo is part of the domestic dog phylogeny and was founded from a small population of domestic dogs. We have also for the first time investigated the dog population on Madagascar and our data strongly indicates a mainland African origin for the Madagascan dogs. Finally, we have investigated the American dog population sampled from throughout the continent and also for the first time included putative indigenous breed dogs such as Chihuahua and Pero Sín Pelo del Peru, and the free-ranging Carolina dogs from southern USA. Our data clearly indicates a primarily Old World origin for the indigenous breed dogs and also for the free-ranging Carolina dogs in USA. We can also for the first time present evidence for continuity between the ancient and extant dog population with e.g. exclusive sharing of a haplotype between a modern sample of Chihuahua and an ancient Mexican sample.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2012. 66 p.
Series
Trita-BIO-Report, ISSN 1654-2312 ; 2012:6
Keyword
mtDNA, domestication, Y-chromosome, SNP, ASY, dog, dingo
National Category
Other Industrial Biotechnology
Identifiers
urn:nbn:se:kth:diva-94145 (URN)978-91-7501-364-0 (ISBN)
Public defence
2012-05-25, Lennart Nilsson-salen, Nobels väg 15A, Solna, 13:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council
Note
QC 20120510Available from: 2012-05-10 Created: 2012-05-08 Last updated: 2012-05-10Bibliographically approved
2. Molecular Profiling of the Population Dynamics: Foundation and Expansion of an Archaic Domesticate
Open this publication in new window or tab >>Molecular Profiling of the Population Dynamics: Foundation and Expansion of an Archaic Domesticate
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

"An ‘exponential growth of science’ throughout modern history has been frequently boasted by numerous narcissistic accounts of ‘modern humanity.’ Nonetheless, ‘modern science’ seems to have overwhelmingly compromised on its original promises by fitting into an ‘industrial scheme.’ With this concern, ‘molecular phylogeographics with conservational ambitions’ would look an intact ground for research efforts in a ‘school of biotechnology.’ The dog (Canis familiaris) as an earliest domestic animal has a history of conflicts over its origins and dispersal. Having those disputes addressed, valuable knowledge could be acquired on the nature and dynamics of domestication, and of human societies particularly of pre-agricultural ages. We employed two most widely-used genealogical markers, the mitochondrial DNA (mtDNA) and the non-recombining portion of the Y-chromosome (NRY), to address dog demography. Through 582 bps of mtDNA Control Region, complemented with whole mitochondrial genomes, it was established that almost all maternal lineages of the domestic dog worldwide coalesce to a population of at least 51 and perhaps many more female wolves in Asia South of Yangtze River (ASY) approximately 16,000 years before present (BP). This was based on the presence of a maximal diversity in this area, a descending gradient of diversity outward it, and a ubiquitous population structure everywhere in the world. A closer examination of this portrait in Southwest Asia (SwAsia) and the Fertile Crescent (FC), a region which has supplied persuasive evidence on early presence of the domestic dog, retrieved the same information, with implications for backbreeding with the local wolf population. Meanwhile, analyses of mtDNA dispersal showed that dogs took the long way via land to Madagascar Island, and not together with humans via sea. By the other approach, the NRY data in 14,437 bps length supplemented the mtDNA in reporting the height of diversity from ASY with a founding population of at least 13 male wolves, but expectably produced lower inter-regional differentiation by diversity. Screening of NRY by a SNP assay in the dingoes of Australia Island as a population of feral dogs revealed restricted and similar dispersal patterns for sires and dams. Prospects of ancient, multilocus and whole genome assays with the emerging high-throughput technologies has still more to promise on finer elaborations of these issues."

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2012. 47 p.
Series
Trita-BIO-Report, ISSN 1654-2312 ; 2012:7
Keyword
Dog, wolf, dingo, mtDNA, NRY, SNP, Madagascar, Australia, domestication
National Category
Genetics
Identifiers
urn:nbn:se:kth:diva-95688 (URN)978-91-7501-349-7 (ISBN)
Public defence
2012-06-08, Hilarp hörsal, Retzius väg 8, Solna, 13:00 (English)
Opponent
Supervisors
Funder
Knut and Alice Wallenberg FoundationSwedish Research Council
Note

QC 20120529

Available from: 2012-05-29 Created: 2012-05-28 Last updated: 2013-09-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Authority records BETA

Savolainen, Peter

Search in DiVA

By author/editor
Ardalan, ArmanOskarsson, MattiasNatanaelsson, ChristianAhmadian, AfshinSavolainen, Peter
By organisation
Gene Technology
In the same journal
Genetica
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 112 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf