Activated Paper Surfaces for the Rapid Hybridization of DNA through Capillary Transport
2012 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 84, no 7, 3311-3317 p.Article in journal (Refereed) Published
The development of low-cost, accurate, and equipment-free diagnostic tests is crucial to many clinical, laboratory, and field applications, including forensics and medical diagnostics. Cellulose fiber-based paper is an inexpensive, biodegradable, and renewable resource, the use of which as a biomolecule detection matrix and support confers several advantages compared to traditional materials such as glass. In this context, a new, facile method for the preparation of surface functionalized papers bearing single-stranded probe DNA (ssDNA) for rapid target hybridization via capillary transport is presented. Optimized reaction conditions were developed that allowed the direct, one-step activation of standard laboratory filters by the inexpensive and readily available bifunctional linking reagent, 1,4-phenylenediisothiocyanate. Such papers were thus amenable to subsequent coupling of amine-labeled ssDNA under standard conditions widely used for glass-based supports. The intrinsic wicking ability of the paper matrix facilitated rapid sample elution through arrays of probe DNA, leading to significant, detectable hybridization in the time required for the sample liquid to transit the vertical length of the strip (less than 2 min). The broad applicability of these paper test strips as rapid and specific diagnostics in "real-life" situations was exemplified by the discrimination of amplicons generated from canine and human mitochondrial and genomic DNA in mock forensic samples.
Place, publisher, year, edition, pages
2012. Vol. 84, no 7, 3311-3317 p.
Bioactive Paper, Visual Dna, Cellulose, Xyloglucan, Biosensors, Aptamers, Assay
IdentifiersURN: urn:nbn:se:kth:diva-95103DOI: 10.1021/ac300025vISI: 000302829800039PubMedID: 22369042ScopusID: 2-s2.0-84859392980OAI: oai:DiVA.org:kth-95103DiVA: diva2:527415
FunderSwedish Research CouncilKnut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201506292012-05-212012-05-142015-06-29Bibliographically approved