Photosensitizer encapsulated organically modified silica nanoparticles for direct two-photon photodynamic therapy and In Vivo functional imaging
2012 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 33, no 19, 4851-4860 p.Article in journal (Refereed) Published
Nanoparticle-assisted two-photon imaging and near infrared (NIR) imaging are two important technologies in biophotonics research. In the present paper, organically modified silica (ORMOSIL) nanoparticles encapsulated with either PpIX (protoporphyrin IX) photosensitizers or IR-820 NIR fluorophores were synthesized and optically characterized. Using the former ORMOSIL nanoparticles, we showed: (i) direct excitation of the fluorescence of PpIX through its efficient two-photon absorption in the intracellular environment of tumor cells, and (ii) cytotoxicity towards tumor cells by PpIX under two-photon irradiation. The latter ORMOSIL nanoparticles can be used as efficient NIR fluorescent contrast agents for various types . in vivo animal imaging. We applied IR-820 doped ORMOSIL nanoparticles in . in vivo brain imaging of mice. We also demonstrated the applications of them to sentinel lymph node (SLN) mapping of mice. Finally, we showed that the nanoprobes could target the subcutaneously xenografted tumor of a mouse for long time observations. ORMOSIL nanoparticles have great potentials for disease diagnosis and clinical therapies.
Place, publisher, year, edition, pages
2012. Vol. 33, no 19, 4851-4860 p.
Fluorescence, In vivo imaging, NIR, ORMOSIL nanoparticles, Photodynamic therapy, Two-photon, Bio photonics, Brain imaging, Clinical therapy, Direct excitation, Disease diagnosis, Fluorescent contrast agents, Functional imaging, In-vivo, In-vivo animal imaging, In-Vivo imaging, Near-infrared imaging, Organically modified silica, ORMOSIL, Protoporphyrin IX, Sentinel lymph nodes, Tumor cells, Two photon, Two photon imaging, Two-photon absorptions, Two-photon photodynamic therapies, Diagnosis, Disease control, Infrared devices, Mammals, Nanoparticles, Photonics, Photons, Photosensitizers, Silica, Tumors, Two photon processes, Synthesis (chemical), contrast medium, macrogol, nanoparticle, protoporphyrin, silicon dioxide, absorption, animal experiment, animal tissue, article, cytotoxicity, encapsulation, human, human cell, irradiation, light absorption, male, mouse, nanoprobe, neuroimaging, nonhuman, priority journal, sentinel lymph node, synthesis, tumor cell, xenograft, Animalia, Mus
Other Electrical Engineering, Electronic Engineering, Information Engineering
IdentifiersURN: urn:nbn:se:kth:diva-96036DOI: 10.1016/j.biomaterials.2012.02.053ISI: 000303966100009ScopusID: 2-s2.0-84859881194OAI: oai:DiVA.org:kth-96036DiVA: diva2:529381
QC 201205302012-05-302012-05-302013-11-19Bibliographically approved