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Direct Measurement of Glyconanoparticles and Lectin Interactions by Isothermal Titration Calorimetry
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
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2012 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 84, no 10, 4248-4252 p.Article in journal (Refereed) Published
Abstract [en]

Glyconanomaterials have shown high potential in applications including bioanalysis and nanomedicine. Here, a quantitative analytical technique, based on isothermal titration calorimetry, was developed to characterize the interactions between glyconanoparticles and lectins. By titrating lectins into the glyconanoparticle solution, the apparent dissociation constant, thermodynamic parameters, and the number of binding sites were derived simultaneously. For the glyconanoparticles-lectin binding pairs investigated, a 3-5 order of magnitude affinity enhancement over the free ligand-lectin interactions was observed which can be attributed to the multivalent ligand presentation on the nanoparticles. The impact of ligand density was also studied, and results showed that the affinity increased with the number of glycans on the nanoparticle.

Place, publisher, year, edition, pages
2012. Vol. 84, no 10, 4248-4252 p.
Keyword [en]
Bioanalysis, Direct measurement, Dissociation constant, Glycans, High potential, Isothermal titration calorimetry, Ligand density, Multivalent ligands, Quantitative analytical technique, Thermodynamic parameter
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-96723DOI: 10.1021/ac3006632ISI: 000303965500002Scopus ID: 2-s2.0-84861134202OAI: oai:DiVA.org:kth-96723DiVA: diva2:532882
Note
QC 20120612Available from: 2012-06-12 Created: 2012-06-11 Last updated: 2017-12-07Bibliographically approved

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