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Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
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2012 (English)In: Oxygen Transport to Tissue XXXIII / [ed] Wolf, M; Bucher, HU; Rudin, M; VanHuffel, S; Wolf, U; Bruley, DF; Harrison, DK, Springer Science+Business Media B.V., 2012, Vol. 737, 263-268 p.Conference paper, Published paper (Refereed)
Abstract [en]

Triplet imaging is a novel optical technique that allows investigating oxygen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolutions which are required for the monitoring of fast kinetics of oxygen concentration in living cells. Calibration and validation are demonstrated with a titration experiment using l-ascorbic acid with the enzyme ascorbase oxidase. The method was applied to a biological cell system, employing as reporter a cytosolic fusion protein of β-galactosidase with a SNAP-tag labeled with tetramethylrhodamine. Oxygen consumption in single smooth muscle cells A7r5 during an [Arg8]-vasopressin- induced contraction is measured. The triplet lifetime images over time can be related to an intracellular oxygen consumption corresponding to a mono-exponentially decaying intracellular oxygen concentration. This is in good agreement with previously reported measurements of oxygen consumption in skeletal muscle fibers.

Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2012. Vol. 737, 263-268 p.
Series
Advances in Experimental Medicine and Biology, ISSN 0065-2598 ; 737
Keyword [en]
ascorbic acid, oxygen, tetramethylrhodamine, animal cell, article, calibration, camera, chemical labeling, controlled study, equipment design, fluorescence imaging, microscopy, molecular imaging, muscle excitation, nonhuman, optical resolution, oxygen concentration, oxygen consumption, priority journal, process monitoring, rat, signal detection, smooth muscle contraction, smooth muscle fiber, triplet imaging
National Category
Condensed Matter Physics
Identifiers
URN: urn:nbn:se:kth:diva-97767DOI: 10.1007/978-1-4614-1566-4_39ISI: 000313161700040Scopus ID: 2-s2.0-84856248504ISBN: 978-146141565-7 (print)OAI: oai:DiVA.org:kth-97767DiVA: diva2:534565
Conference
38th Annual Conference of the International-Society-on-Oxygen-Transport-to-Tissue (ISOTT),Centro Stefano Franscini, ETH Zurich, Ascona, SWITZERLAND,JUL 18-23, 2010
Note

QC 20120618

Available from: 2012-06-18 Created: 2012-06-14 Last updated: 2013-02-08Bibliographically approved

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