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Specific Evolution of F-1-Like ATPases in Mycoplasmas
Univ Bordeaux, Villenave Dornon, France ; INRA Villenave Dornon, France .
Univ Bordeaux, Villenave Dornon, France ; INRA Villenave Dornon, France .
Univ Bordeaux, Bordeaux, France ; IBMC, CNRS, Bordeaux, France.
Univ Bordeaux, Villenave Dornon, France ; INRA Villenave Dornon, France .
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2012 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, no 6, e38793- p.Article in journal (Refereed) Published
Abstract [en]

F1F0 ATPases have been identified in most bacteria, including mycoplasmas which have very small genomes associated with a host-dependent lifestyle. In addition to the typical operon of eight genes encoding genuine F1F0 ATPase (Type 1), we identified related clusters of seven genes in many mycoplasma species. Four of the encoded proteins have predicted structures similar to the alpha, beta, gamma and e subunits of F-1 ATPases and could form an F-1-like ATPase. The other three proteins display no similarity to any other known proteins. Two of these proteins are probably located in the membrane, as they have three and twelve predicted transmembrane helices. Phylogenomic studies identified two types of F-1-like ATPase clusters, Type 2 and Type 3, characterized by a rapid evolution of sequences with the conservation of structural features. Clusters encoding Type 2 and Type 3 ATPases were assumed to originate from the Hominis group of mycoplasmas. We suggest that Type 3 ATPase clusters may spread to other phylogenetic groups by horizontal gene transfer between mycoplasmas in the same host, based on phylogeny and genomic context. Functional analyses in the ruminant pathogen Mycoplasma mycoides subsp. mycoides showed that the Type 3 cluster genes were organized into an operon. Proteomic analyses demonstrated that the seven encoded proteins were produced during growth in axenic media. Mutagenesis and complementation studies demonstrated an association of the Type 3 cluster with a major ATPase activity of membrane fractions. Thus, despite their tendency toward genome reduction, mycoplasmas have evolved and exchanged specific F-1-like ATPases with no known equivalent in other bacteria. We propose a model, in which the F-1-like structure is associated with a hypothetical X-0 sector located in the membrane of mycoplasma cells.

Place, publisher, year, edition, pages
2012. Vol. 7, no 6, e38793- p.
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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
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URN: urn:nbn:se:kth:diva-99079DOI: 10.1371/journal.pone.0038793ISI: 000305351700070Scopus ID: 2-s2.0-84862023527OAI: oai:DiVA.org:kth-99079DiVA: diva2:541542
Note
QC 20120719Available from: 2012-07-19 Created: 2012-07-13 Last updated: 2017-12-07Bibliographically approved

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