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Small RNA fragments in complex culture media cause alterations in protein profiles of three species of bacteria
KTH, School of Biotechnology (BIO), Environmental Microbiology (closed September 2009).
2012 (English)In: BioTechniques, ISSN 0736-6205, E-ISSN 1940-9818, Vol. 52, no 3, 167-172 p.Article in journal (Refereed) Published
Abstract [en]

Efforts to delineate the basis for variations in protein profiles of different membrane fractions from various bacterial pathogens led to the finding that even the same medium [e.g., Luria Bertani (LB) broth] purchased from different commercial sources generates remarkably dissimilar protein profiles despite similar growth characteristics. Given the pervasive roles small RNAs play in regulating gene expression, we inquired if these source-specific differences due to media arise from disparities in the presence of small RNAs. Indeed, LB media components from two different commercial suppliers contained varying, yet significant, amounts of 10-80 bp small RNAs. Removal of small RNA from LB using RNaseA during media preparation resulted in significant changes in bacterial protein expression profiles. Our studies underscore the fact that seemingly identical growth media can lead to dramatic alterations in protein expression patterns, highlighting the importance of utilizing media free of small RNA during bacteriological studies. Finally, these results raise the intriguing possibility that similar pools of small RNAs in the environment can influence bacterial adaptation.

Place, publisher, year, edition, pages
2012. Vol. 52, no 3, 167-172 p.
Keyword [en]
Luria bertani broth, Protein expression, RNA-depletion, Small RNA, Subcellular proteins, bacterial protein, protein variant, RNA, article, bacterium culture, controlled study, culture medium, Erwinia, gene expression regulation, genomic fragment, mutation, nonhuman, Pseudomonas aeruginosa, Vibrio cholerae, Vibrio harveyi
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-99833ISI: 000302747500009Scopus ID: 2-s2.0-84858138505OAI: oai:DiVA.org:kth-99833DiVA: diva2:542553
Note

QC 20120802

Available from: 2012-08-02 Created: 2012-08-02 Last updated: 2017-06-15Bibliographically approved

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Pavankumar, Asalapuram Ramachand
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