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Single-cell culture in microwells
KTH, School of Biotechnology (BIO), Nano Biotechnology.
2012 (English)In: Single-Cell Analysis, Springer Science+Business Media B.V., 2012, Vol. 853, 41-52 p.Chapter in book (Refereed)
Abstract [en]

In order to better understand cellular processes and behavior, a controlled way of studying high numbers of single cells and their clone formation is greatly needed. This chapter describes a microwell plate with 672 wells in a standard array/slide format, applied for single-cell culture and analysis. Single cells can be seeded into each well of the plate (1) manually or (2) automatically using a sorting flow cytometer, followed by week-long culture and detection of cell growth, protein expression, etc. The glass/silicon plate is compatible with most standard instrumentation to facilitate easy handling and enable use of the plate for single-cell analysis in most laboratory settings.

Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2012. Vol. 853, 41-52 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 853
Keyword [en]
Array, Cell culture, Clone formation, FACS, Long-term analysis, Microwell, Plate, Single cell
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-99772DOI: 10.1007/978-1-61779-567-1_5Scopus ID: 2-s2.0-84858423561ISBN: 978-161779566-4 OAI: oai:DiVA.org:kth-99772DiVA: diva2:542618
Note
QC 20120802Available from: 2012-08-02 Created: 2012-08-01 Last updated: 2012-08-02Bibliographically approved

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