Single-cell culture in microwells
2012 (English)In: Single-Cell Analysis, Springer Science+Business Media B.V., 2012, Vol. 853, 41-52 p.Chapter in book (Refereed)
In order to better understand cellular processes and behavior, a controlled way of studying high numbers of single cells and their clone formation is greatly needed. This chapter describes a microwell plate with 672 wells in a standard array/slide format, applied for single-cell culture and analysis. Single cells can be seeded into each well of the plate (1) manually or (2) automatically using a sorting flow cytometer, followed by week-long culture and detection of cell growth, protein expression, etc. The glass/silicon plate is compatible with most standard instrumentation to facilitate easy handling and enable use of the plate for single-cell analysis in most laboratory settings.
Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2012. Vol. 853, 41-52 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 853
Array, Cell culture, Clone formation, FACS, Long-term analysis, Microwell, Plate, Single cell
IdentifiersURN: urn:nbn:se:kth:diva-99772DOI: 10.1007/978-1-61779-567-1_5ScopusID: 2-s2.0-84858423561ISBN: 978-161779566-4OAI: oai:DiVA.org:kth-99772DiVA: diva2:542618
QC 201208022012-08-022012-08-012012-08-02Bibliographically approved