Perfusion culture of mammalian cells in a microfluidic channel with a built-in pillar array
2012 (English)In: Single-Cell Analysis, Springer Science+Business Media B.V., 2012, Vol. 853, 83-94 p.Chapter in book (Refereed)
In vitro culture of mammalian cells is fundamental to various biological studies such as single cell analysis, pathological research, and drug/therapy development. However, there are limitations with the current in vitro cell culture methods. Cells tend to lose their specific functions due to the lack of a cellular microenvironment when they are maintained under standard culture conditions. Microscale devices can be a novel tool to reestablish a cellular microenvironment for culturing mammalian cells in vitro and maintaining their differentiated functions. Different microscale cell culture techniques have been developed to suit different biological applications. This chapter describes a method to trap and culture mammalian cells in a microfluidic channel with a built-in pillar array.
Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2012. Vol. 853, 83-94 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 853
3D Cell culture, Cell analysis, Microenvironment, Microfluidics
IdentifiersURN: urn:nbn:se:kth:diva-99770DOI: 10.1007/978-1-61779-567-1_8ScopusID: 2-s2.0-84863388051ISBN: 978-161779566-4OAI: oai:DiVA.org:kth-99770DiVA: diva2:542620
QC 201208022012-08-022012-08-012012-08-02Bibliographically approved