Change search
ReferencesLink to record
Permanent link

Direct link
Antibodies for profiling the human proteome-The Human Protein Atlas as a resource for cancer research
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-8141-8449
2012 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 12, no 13, 2067-2077 p.Article in journal (Refereed) Published
Abstract [en]

In this review, we present an update on the progress of the Human Protein Atlas, with an emphasis on strategies for validating immunohistochemistry-based protein expression patterns and on the possibilities to extend the map of protein expression patterns for cancer research projects. The objectives underlying the Human Protein Atlas include (i) the generation of validated antibodies toward a major isoform of all proteins encoded by the human genome, (ii) creating an information database of protein expression patterns in normal human tissues, in cells, and in cancer, and (iii) utilizing generated antibodies and protein expression data as tools to identify clinically useful biomarkers. The success of such an effort is dependent on the validity of antibodies as specific binders of intended targets in applications used to map protein expression patterns. The development of strategies to support specific target binding is crucial and remains a challenge as a large fraction of proteins encoded by the human genome is poorly characterized, including the approximately one-third of all proteins lacking evidence of existence. Conceivable methods for validation include the use of paired antibodies, i.e. two independent antibodies targeting different and nonoverlapping epitopes on the same protein as well as comparative analysis of mRNA expression patterns with corresponding proteins.

Place, publisher, year, edition, pages
2012. Vol. 12, no 13, 2067-2077 p.
Keyword [en]
Antibody, Biomarker, Biomedicine, Cancer, Protein profile, Tissue staining
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-100501DOI: 10.1002/pmic.201100504ISI: 000306473800002ScopusID: 2-s2.0-84864001338OAI: diva2:543818
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience

QC 20120810

Available from: 2012-08-10 Created: 2012-08-09 Last updated: 2013-04-15Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Schwenk, Jochen M.
By organisation
Proteomics (closed 20130101)Science for Life Laboratory, SciLifeLab
In the same journal
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 50 hits
ReferencesLink to record
Permanent link

Direct link