APELIN promotes hematopoiesis from human embryonic stem cells
2012 (English)In: Blood, ISSN 0006-4971, E-ISSN 1528-0020, Vol. 119, no 26, 6243-6254 p.Article in journal (Refereed) Published
Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein-coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR mAb, were found in both posterior mesoderm and anterior mesendoderm populations and were enriched in hemangioblast colony-forming cells (Bl-CFC). The addition of APLN peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hematoendothelial genes in differentiating hESCs, and increased the frequency of Bl-CFCs by up to 10-fold. Furthermore, APLN peptide also synergized with VEGF to promote the growth of hESC-derived endothelial cells. These studies identified APLN as a novel growth factor for hESC-derived hematopoietic and endothelial cells.
Place, publisher, year, edition, pages
2012. Vol. 119, no 26, 6243-6254 p.
Protein-Coupled Receptor, Caliber Size Regulation, Primitive Streak, Blood-Vessels, In-Vivo, Differentiation Cultures, Definitive Endoderm, Endothelial-Cells, Xenopus-Laevis, Expression
IdentifiersURN: urn:nbn:se:kth:diva-101740DOI: 10.1182/blood-2011-12-396093ISI: 000307400700015ScopusID: 2-s2.0-84863514081OAI: oai:DiVA.org:kth-101740DiVA: diva2:548980
QC 201209032012-09-032012-09-032012-09-03Bibliographically approved