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Stepping stones in DNA sequencing
KTH, School of Biotechnology (BIO), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-4313-1601
2012 (English)In: Biotechnology Journal, ISSN 1860-6768, E-ISSN 1860-7314, Vol. 7, no 9, 1063-1073 p.Article, review/survey (Refereed) Published
Abstract [en]

In recent years there have been tremendous advances in our ability to rapidly and cost-effectively sequence DNA. This has revolutionized the fields of genetics and biology, leading to a deeper understanding of the molecular events in life processes. The rapid technological advances have enormously expanded sequencing opportunities and applications, but also imposed strains and challenges on steps prior to sequencing and in the downstream process of handling and analysis of these massive amounts of sequence data. Traditionally, sequencing has been limited to small DNA fragments of approximately one thousand bases (derived from the organism's genome) due to issues in maintaining a high sequence quality and accuracy for longer read lengths. Although many technological breakthroughs have been made, currently the commercially available massively parallel sequencing methods have not been able to resolve this issue. However, recent announcements in nanopore sequencing hold the promise of removing this read-length limitation, enabling sequencing of larger intact DNA fragments. The ability to sequence longer intact DNA with high accuracy is a major stepping stone towards greatly simplifying the downstream analysis and increasing the power of sequencing compared to today. This review covers some of the technical advances in sequencing that have opened up new frontiers in genomics.

Place, publisher, year, edition, pages
2012. Vol. 7, no 9, 1063-1073 p.
Keyword [en]
DNA, Genomics, Nanopore sequencing, Parallel sequencing, Sequencing
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-103135DOI: 10.1002/biot.201200153ISI: 000308475100008Scopus ID: 2-s2.0-84866041323OAI: oai:DiVA.org:kth-103135DiVA: diva2:559360
Funder
Swedish Research CouncilScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Available from: 2012-10-09 Created: 2012-10-04 Last updated: 2017-12-07Bibliographically approved

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