Towards subcellular localization of the human proteome using bioimaging
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. The objective of the studies presented in this doctoral thesis was to systematically explore the human proteome at the subcellular level using bioimaging and to develop techniques for validation of the results obtained.
A common imaging technique for protein detection is immunofluorescence (IF), where antibodies are used to target proteins in fixated cells. A fixation protocol suitable for large-scale IF studies was developed and optimized to work for a broad set of proteins. As the technique relies on antibodies, validation of their specificity to the target protein is crucial. A platform based on siRNA gene silencing in combination with IF was set-up to evaluate antibody specificity by quantitative image analysis before and after suppression of its target protein. As a proof of concept, the platform was then used for validation of 75 antibodies, proving it to be applicable for validation of antibodies in a systematic manner.
Because of the fixation, there is a common concern about how well IF data reflects the in vivo subcellular distribution of proteins. To address this, 500 proteins were tagged with green fluorescent protein (GFP) and used to compare protein localization results between IF to those achieved using GFP tagged proteins in live cells. It was concluded that protein localization data from fixated cells satisfactory represented the situation in vivo and together exhibit a powerful approach for confirming localizations of yet uncharacterized proteins.
Finally, a global analysis based on IF data of approximately 20 % of the human proteome was performed, providing a first overview of the subcellular landscape in three different cell lines. It was found that the intracellular distribution of proteins is complex, with many proteins occurring in several organelles. The results also confirmed the close relationship between protein function and localization, which in a way further strengthens the accuracy of the IF approach for detection of proteins at the subcellular level.
Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2012. , 60 p.
Trita-BIO-Report, ISSN 1654-2312 ; 12:19
Antibody, antibody validation, automated image analysis, automated microscopy, cell line, confocal microscopy, fixation, green fluorescent protein (GFP), immunofluorescence (IF), organelle, protein expression, siRNA, subcellular localization
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-103616ISBN: 978-91-7501-483-8OAI: oai:DiVA.org:kth-103616DiVA: diva2:560974
2012-11-09, MTC, Karolinska institutet, Stockholm, 10:00 (English)
Wählby, Carolina, Docent
Lundberg, Emma, Associate Professor
ProjectsThe Human Protein Atlas
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201210172012-10-172012-10-162013-04-15Bibliographically approved
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