Development of a novel microfluidic device for long-term in situ monitoring of live cells in 3-dimensional matrices
2012 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 14, no 5, 885-893 p.Article in journal (Refereed) Published
Using the latest innovations in microfabrication technology, 3-dimensional microfluidic cell culture systems have been developed as an attractive alternative to traditional 2-dimensional culturing systems as a model for long-term microscale cell-based research. Most microfluidic systems are based on the embedding of cells in hydrogels. However, physiologically realistic conditions based on hydrogels are difficult to obtain and the systems are often too complicated. We have developed a microfluidic cell culture device that incorporates a biodegradable rigid 3D polymer scaffold using standard soft lithography methods. The device permits repeated high-resolution fluorescent imaging of live cell populations within the matrix over a 4 week period. It was also possible to track cell development at the same spatial location throughout this time. In addition, human primary periodontal ligament cells were induced to produce quantifiable calcium deposits within the system. This simple and versatile device should be readily applicable for cell-based studies that require long-term culture and high-resolution bioimaging.
Place, publisher, year, edition, pages
2012. Vol. 14, no 5, 885-893 p.
Microfabrication, Soft lithography, Live cell imaging, 3-dimensional cell culture
Other Medical Engineering
IdentifiersURN: urn:nbn:se:kth:diva-103760DOI: 10.1007/s10544-012-9668-1ISI: 000308963200009ScopusID: 2-s2.0-84870298439OAI: oai:DiVA.org:kth-103760DiVA: diva2:563087
FunderEU, FP7, Seventh Framework Programme, 242175-VascuBoneScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201210292012-10-292012-10-192013-04-15Bibliographically approved