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Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).ORCID iD: 0000-0003-1899-7649
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-8993-048X
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).ORCID iD: 0000-0002-9977-5724
2012 (English)In: Scientific Reports, ISSN 2045-2322, Vol. 2, 706- p.Article in journal (Refereed) Published
Abstract [en]

As antibody-based diagnosis and therapy grow at an increased pace, there is a need for methods which rapidly and accurately determine antibody-antigen interactions. Here, we report a method for the multiplex determination of antibody epitopes using bacterial cell-surface display. A protein-fragment library with 107 cell clones, covering 60 clinically-relevant protein targets, was created and characterized with massively parallel sequencing. Using this multi-target fragment library we determined simultaneously epitopes of commercial monoclonal and polyclonal antibodies targeting PSMA, EGFR, and VEGF. Off-target binding was observed for one of the antibodies, which demonstrates the method's ability to reveal cross-reactivity. We exemplify the detection of structural epitopes by mapping the therapeutic antibody Avastin. Based on our findings we suggest this method to be suitable for mapping linear and structural epitopes of monoclonal and polyclonal antibodies in a multiplex fashion and could find applicability in serum profiling as well as other protein-protein interaction studies.

Place, publisher, year, edition, pages
2012. Vol. 2, 706- p.
Keyword [en]
Growth-Factor Receptor, Cell-Surface, Staphylococcus-Carnosus, Recombinant Proteins, Crystal-Structure, Flow-Cytometry, Binding Site, Antibodies, Fab, Antigen
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-104361DOI: 10.1038/srep00706ISI: 000309524900004Scopus ID: 2-s2.0-84874551743OAI: oai:DiVA.org:kth-104361DiVA: diva2:564789
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20121105

Available from: 2012-11-05 Created: 2012-11-01 Last updated: 2012-11-05Bibliographically approved

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Hudson, Elton P.Uhlén, Mathias

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