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High average brightness water window source for short-exposure cryomicroscopy
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.ORCID iD: 0000-0001-8604-735X
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
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2012 (English)In: Optics Letters, ISSN 0146-9592, E-ISSN 1539-4794, Vol. 37, no 21, 4425-4427 p.Article in journal (Refereed) Published
Abstract [en]

Laboratory water window cryomicroscopy has recently demonstrated similar image quality as synchrotron-based microscopy but still with much longer exposure times, prohibiting the spread to a wider scientific community. Here we demonstrate high-resolution laboratory water window imaging of cryofrozen cells with 10 s range exposure times. The major improvement is the operation of a lambda = 2.48 nm, 2 kHz liquid nitrogen jet laser plasma source with high spatial and temporal stability at high average brightness >1.5 x 10(12) ph/(s x sr x mu m(2) x line), i.e., close to that of early synchrotrons. Thus, this source enables not only biological x-ray microscopy in the home laboratory but potentially other applications previously only accessible at synchrotron facilities.

Place, publisher, year, edition, pages
Optical Society of America, 2012. Vol. 37, no 21, 4425-4427 p.
Keyword [en]
X-Ray Microscopy
National Category
Other Physics Topics
URN: urn:nbn:se:kth:diva-107087DOI: 10.1364/OL.37.004425ISI: 000310577700024ScopusID: 2-s2.0-84868308552OAI: diva2:575179
Swedish Research CouncilSwedish Foundation for Strategic Research

QC 20121207

Available from: 2012-12-07 Created: 2012-12-06 Last updated: 2016-03-24Bibliographically approved
In thesis
1. 3D X-ray microscopy: image formation, tomography and instrumentation
Open this publication in new window or tab >>3D X-ray microscopy: image formation, tomography and instrumentation
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Tomography in soft X-ray microscopy is an emerging technique for obtaining quantitative 3D structural information about cells. One of its strengths, compared with other techniques, is that it can image intact cells in their near-native state at a few 10 nm’s resolution, without staining. However, the methods for reconstructing 3D-data rely on algorithms that assume projection data, which the images are generally not due to the imaging systems’ limited depth of focus. To bring out the full potential of tomography in soft X-ray microscopy an improved understanding of the image formation is desired.

This Thesis reviews zone plate-based X-ray microscopy for biological imaging and the theory necessary for a numerical implementation of a 3D image formation model. Furthermore, a novel reconstruction approach is proposed that improves the overall resolution in a reconstruction of a tomographically imaged object. This is demonstrated by simulations and experiments. Finally, this Thesis covers work on the Stockholm X-ray microscope, including an upgrade of the X-ray source yielding unprecedented brightness for a compact system. With this upgrade it was possible to do high-quality imaging of cells in their near-native state with only 10 second exposures.

Abstract [sv]

Tomografi i mjukröntgenmikroskopi är en ny teknik för att få ut kvantitativ strukturell 3D information om celler. Dess styrka jämfört med andra tekniker är att den kan avbilda intakta celler i deras nära naturliga tillstånd med ett par 10 nm upplösning, utan omfattande preparering. Dock är metoderna för att rekonstruera 3D-data beroende av algoritmer som antar projektionsdata, vilket bilderna i allmänhet inte är på grund av avbildningsystemens begränsade skärpedjup. För att få ut den fulla potentialen av tomografi i röntgenmikroskopi behövs en ökad förståelse för avbildningsprocessen.

Denna avhandling behandlar zonplatte-baserad röntgenmikroskopi för biologisk avbildning och den nödvändiga teorin för en numerisk implementering av en avbildningsmodell i 3D. En ny rekonstruktionsmetod föreslås som förbättrar upplösningen i rekonstruktionen för ett tomografiskt avbildat objekt. Detta visas i simuleringar och experiment. Slutligen omfattar denna avhandling arbete på Stockholms mjukröntgenmikroskop, inklusive en uppgradering av röntgenkällan som ger oöverträffad ljusstyrka för ett kompakt system. Denna uppgradering möjliggör högkvalitativ avbildning av celler i deras nästan naturliga tillstånd med endast 10 sekunders exponering.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2016. viii, 75 p.
TRITA-FYS, ISSN 0280-316X ; 2016:15
X-ray microscopy, image formation theory, partial coherence in imaging, wave propagation, tomography, instrumentation
National Category
Physical Sciences
Research subject
urn:nbn:se:kth:diva-184095 (URN)978-91-7595-914-6 (ISBN)
Public defence
2016-04-22, FD5, Albanova universitetscentrum, Roslagstullsbacken 21, Stockholm, 13:00 (English)
Swedish Research CouncilKnut and Alice Wallenberg Foundation

QC 20160324

Available from: 2016-03-24 Created: 2016-03-23 Last updated: 2016-03-24Bibliographically approved

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Martz, Dale H.Selin, Mårtenvon Hofsten, OlovFogelqvist, EmelieHolmberg, AndersVogt, UlrichHertz, Hans M.
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