High average brightness water window source for short-exposure cryomicroscopy
2012 (English)In: Optics Letters, ISSN 0146-9592, E-ISSN 1539-4794, Vol. 37, no 21, 4425-4427 p.Article in journal (Refereed) Published
Laboratory water window cryomicroscopy has recently demonstrated similar image quality as synchrotron-based microscopy but still with much longer exposure times, prohibiting the spread to a wider scientific community. Here we demonstrate high-resolution laboratory water window imaging of cryofrozen cells with 10 s range exposure times. The major improvement is the operation of a lambda = 2.48 nm, 2 kHz liquid nitrogen jet laser plasma source with high spatial and temporal stability at high average brightness >1.5 x 10(12) ph/(s x sr x mu m(2) x line), i.e., close to that of early synchrotrons. Thus, this source enables not only biological x-ray microscopy in the home laboratory but potentially other applications previously only accessible at synchrotron facilities.
Place, publisher, year, edition, pages
Optical Society of America, 2012. Vol. 37, no 21, 4425-4427 p.
Other Physics Topics
IdentifiersURN: urn:nbn:se:kth:diva-107087DOI: 10.1364/OL.37.004425ISI: 000310577700024ScopusID: 2-s2.0-84868308552OAI: oai:DiVA.org:kth-107087DiVA: diva2:575179
FunderSwedish Research CouncilSwedish Foundation for Strategic Research
QC 201212072012-12-072012-12-062016-03-24Bibliographically approved