Coagulant proteins identified in Mustard: a potential water treatment agent
2014 (English)In: International Journal of Environmental Science and Technology, ISSN 1735-1472, Vol. 11, no 4, 873-880 p.Article in journal (Refereed) Published
The use of natural coagulant protein in drinking water treatment has been discussed for a long time, though the method is still not in practice, probably due to limited knowledge and availability of material. In the present work, different Mustard varieties were tested for the presence of coagulant protein compared with Moringa seed extract and their potential application in water treatment. The coagulation activity of the protein extract was measured using synthetic clay solution as well as water from pond. The protein content was determined by Bradford method, molecular mass determined by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and peptide sequence was analyzed by Mass spectrometry. Extract of Mustard (large) and Moringa seed showed coagulation activity of a parts per thousand...70 and a parts per thousand...85 % after 90 min, respectively. Interestingly, seed extracts from other Mustard varieties had coagulation activity after heat activation at 95 A degrees C for 5 h. However, the coagulation activity of Mustard seed extract against turbid pond water was higher (a parts per thousand...60 %) compared to Moringa seed extract (a parts per thousand...50 %). The peptide sequence analysis of 6.5 and 9 kDa proteins was found to be homologous to Moringa coagulant protein and napin3, respectively. To our knowledge, this could be the first report on Mustard seed having coagulant protein. The coagulation activity of Mustard (large) against highly turbid pond water suggested that it could be a potential natural coagulant for water treatment.
Place, publisher, year, edition, pages
2014. Vol. 11, no 4, 873-880 p.
Brassica, Napin, Moringa coagulant protein, Peptide sequence, Thermo-tolerant
IdentifiersURN: urn:nbn:se:kth:diva-107283DOI: 10.1007/s13762-013-0282-4ISI: 000334172200001ScopusID: 2-s2.0-84897519615OAI: oai:DiVA.org:kth-107283DiVA: diva2:575547
FunderSida - Swedish International Development Cooperation Agency, SWE-2007-401
QC 20140516. Updated from manuscript to article in journal.2012-12-102012-12-102014-05-16Bibliographically approved