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Antibacterial activity of Mustard and Moringa seed extracts against pathogenic organisms
Anna University, Chennai.
KTH, School of Biotechnology (BIO), Environmental Microbiology.
Anna University, Chennai.
KTH, School of Biotechnology (BIO), Environmental Microbiology.
(English)Manuscript (preprint) (Other academic)
National Category
Environmental Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-107334OAI: oai:DiVA.org:kth-107334DiVA: diva2:575548
Note

QS 2012

Available from: 2012-12-10 Created: 2012-12-10 Last updated: 2012-12-14Bibliographically approved
In thesis
1. Coagulant Protein from plant materials: Potential Water Treatment Agent
Open this publication in new window or tab >>Coagulant Protein from plant materials: Potential Water Treatment Agent
2013 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Access to fresh water is a human right, yet more than 780 million people, especially in rural areas, rely on unimproved sources and the need for finding ways of treating water is crucial. Although the use of natural coagulant protein in drinking water treatment has been discussed for a long time, the method is still not in practice, probably due to availability of material and limited knowledge. In this study, about hundred different crude extracts made from plant materials found in Southern India were screened for coagulation activity. Extracts of three Brassica species (Mustard, Cabbage and Cauliflower) were showing activity comparable to that of Moringa oleifera and were further investigated. Their protein content and profile were compared against each other and with coagulant protein from Moringa. Mustard (large) and Moringa seed proteins were also studied for their effect against clinically isolated bacterial strains. The protein profiles of Brassica extract showed predominant bands around 9kDa and 6.5kDa by SDS-PAGE. The peptide sequence analysis of Mustard large identified the 6.5kDa protein as Moringa coagulant protein (MO2.1) and the 9kDa protein band as seed storage protein napin3. Of thirteen clinical strains analysed, Moringa and Mustard large were proven effective in either aggregation activity or growth kinetic method or both in all thirteen and nine strains respectively. To my knowledge this is the first report on the presence of coagulant protein in Brassica seeds. Owing to the promising results Brassica species could possibly be used as a substitute to Moringa coagulating agent and chemicals in drinking water treatment. 

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2013. 37 p.
Series
Trita-BIO-Report, ISSN 1654-2312 ; 2013:1
Keyword
antimicrobial effect; Brassica; coagulant protein; MO2.1; Moringa oleifera; Mustard; napin; thermo-resistance; plant material; protein extraction; salt extract.
National Category
Environmental Biotechnology Water Treatment
Identifiers
urn:nbn:se:kth:diva-107335 (URN)978-91-7501-593-4 (ISBN)
Presentation
2013-01-18, FB55, AlbaNova Universitetscentrum, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

QC 20121214

Available from: 2012-12-14 Created: 2012-12-10 Last updated: 2013-09-12Bibliographically approved

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Citation style
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